Borrelia Burgdorferi 抗体 (Peroxidase (POD))
适用: Borrelia burgdorferi
WB, IHC, EIA
宿主: 兔
Polyclonal
Peroxidase (POD)
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- 抗原 See all Borrelia Burgdorferi products
- Borrelia Burgdorferi
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适用
- Borrelia burgdorferi
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Borrelia Burgdorferi antibody is conjugated to Peroxidase (POD)
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Enzyme Immunoassay (EIA)
- 特异性
- Reactive Western Blot bands at 83kD, 41kD, 34kD and 31kD + additional low MW bands
- 交叉反应 （详细）
- Cross-reacts with Treponema pallidum, B. hermsii & B. parkerii
- 纯化方法
- This product consists of purified IgG fraction of the neat antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. . Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.
- 免疫原
- Whole cell preparation from B. burgdorferi
- 亚型
- IgG
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- 应用备注
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TITER : >1:1,000 in Western Blot. >1:40,000 in avidin-biotin IFA
This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays, Functions well in formalin fixed/paraffin embedded settings - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.
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- 抗原
- Borrelia Burgdorferi
- Abstract
- Borrelia Burgdorferi 产品
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