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Occludin 抗体 (AA 282-415)

OCLN 适用: 人 IHC 宿主: 小鼠 Monoclonal MSVA-415M unconjugated
产品编号 ABIN7477543
发货至: 中国
  • 抗原 See all Occludin (OCLN) 抗体
    Occludin (OCLN)
    抗原表位
    • 16
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    AA 282-415
    适用
    • 102
    • 65
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    • 34
    • 18
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    宿主
    • 87
    • 16
    • 3
    • 1
    小鼠
    克隆类型
    • 86
    • 21
    单克隆
    标记
    • 44
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    This Occludin antibody is un-conjugated
    应用范围
    • 86
    • 34
    • 28
    • 26
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    • 14
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    • 11
    • 7
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    Immunohistochemistry (IHC)
    免疫原
    ecombinant fragment of human Occludin protein (around aa 282-415) (exact sequence is proprietary)
    克隆位点
    MSVA-415M
    亚型
    IgG
    Top Product
    Discover our top product OCLN Primary Antibody
  • 应用备注
    IHC 1:100-1:200
    说明

    Positive Control: Colon: A strong membranous occludin staining of epithelial cells should be seen with a particularly strong staining at the apical/luminal membranes.

    Negative Control: Colon: All non-epithelial and non-endothelial cells must not show any occludin staining.

    实验流程
    Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
    限制
    仅限研究用
  • 状态
    Liquid
    储存条件
    4 °C,-20 °C,-80 °C
    储存方法
    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
  • 抗原
    Occludin (OCLN)
    别名
    Occludin (OCLN 产品)
    别名
    AI503564 antibody, Ocl antibody, ocln antibody, oclnb antibody, tpmt antibody, OCLN antibody, wu:fd23h10 antibody, wu:fi13c01 antibody, zgc:113992 antibody, zgc:56359 antibody, BLCPMG antibody, occludin antibody, occludin S homeolog antibody, occludin a antibody, Ocln antibody, OCLN antibody, ocln.S antibody, ocln antibody, oclna antibody
    背景
    BLCPMG, Occludin, OCLN, Phosphatase 1 regulatory subunit 115, PPP1R115, PTORCH1, Tight junction protein occludin,Occludin antibody validated for immunohistochemistry on 76 different Normal Tissues
    UniProt
    Q16625
    途径
    Cell-Cell Junction Organization, Hepatitis C
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