Helicobacter Pylori 抗体 (AA 323-445)
-
- 抗原 See all Helicobacter Pylori (H. pylori) products
- Helicobacter Pylori (H. pylori)
- 抗原表位
- AA 323-445
-
适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This Helicobacter Pylori antibody is un-conjugated
-
应用范围
- Immunohistochemistry (IHC)
- 免疫原
- Recombinant Helicobacter pylori Catalase protein fragment (around aa323-445) (Exact sequence is proprietary)
- 克隆位点
- MSVA-466M
- 亚型
- IgG2b, kappa
-
-
- 应用备注
- IHC 1:100-1:200
- 说明
-
Positive Control: Gastric tissue with known helicobacter pylori infection should show a distinct staining of bacteria on the surface and/or within glands.
Negative Control: Gastric tissue: Normal epithelial and stromal cells should not stain.
- 实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
-
- 抗原
- Helicobacter Pylori (H. pylori)
- 别名
- Helicobacter Pylori (H. pylori 产品)
- 物质类
- Virus
- 背景
- H. pylori,Helicobacter Pylori antibody validated for immunohistochemistry on 76 different Normal Tissues
-