GP2 抗体 (AA 35-179)
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- 抗原 See all GP2 products
- GP2 (Glycoprotein 2 (Zymogen Granule Membrane) (GP2))
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抗原表位
- AA 35-179
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This GP2 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC)
- 免疫原
- Recombinant fragment of human GP2 protein (around aa 35-179) (exact sequence is proprietary)
- 克隆位点
- MSVA-475M
- 亚型
- IgG
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- 应用备注
- IHC 1:100-1:200
- 说明
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Positive Control: Pancreas: Acinar cells should show a strong immunostaining with apical predominance. Duodenum: Glandular cells of Brunner glands should show a weak to moderate GP2 positivity.
Negative Control: Colon: GP2 staining should be completely absent in glandular and stromal cells.
- 实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- 抗原
- GP2 (Glycoprotein 2 (Zymogen Granule Membrane) (GP2))
- 别名
- GP2 (GP2 产品)
- 别名
- ZAP75 antibody, 2310037I18Rik antibody, AV060639 antibody, gp80 antibody, glycoprotein 2 antibody, glycoprotein 2 (zymogen granule membrane) antibody, GP2 antibody, Gp2 antibody
- 物质类
- Viral Protein
- 背景
- Glycoprotein 2 (zymogen granule membrane), GP2, Pancreatic zymogen granule membrane associated protein GP2, Pancreatic zymogen granule membrane protein GP-2, ZAP75,GP2 antibody validated for immunohistochemistry on 76 different Normal Tissues
- UniProt
- P55259
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