Recombinant Keratin 10 抗体
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- 抗原 See all Keratin 10 (KRT10) 抗体
- Keratin 10 (KRT10)
- 抗体类型
- Recombinant Antibody
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Keratin 10 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC)
- 免疫原
- Recombinant full-lenth human KRT10 protein.
- 克隆位点
- MSVA-610M
- 亚型
- IgG
- Top Product
- Discover our top product KRT10 Primary Antibody
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- 应用备注
- IHC 1:100-1:200
- 说明
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Positive Control: Skin: All squamous epithelial cells (except the basal cell layer) should show strong cytoplasmic staining, while the basal cell layer remains KRT10 negative or shows only minimal staining.
Negative Control: Colon: All epithelial, inflammatory, stromal and muscle cells must not show any KRT10 staining.
- 实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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