Recombinant Collagen IV 抗体
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- 抗原 See all Collagen IV (COL4) 抗体
- Collagen IV (COL4) (Collagen, Type IV (COL4))
- 抗体类型
- Recombinant Antibody
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适用
- 人
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宿主
- 兔
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克隆类型
- 单克隆
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标记
- This Collagen IV antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC)
- 免疫原
- Purified human placental extract
- 克隆位点
- MSVA-704R
- 亚型
- IgG
- Top Product
- Discover our top product COL4 Primary Antibody
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- 应用备注
- IHC 1:100-1:200
- 说明
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Positive Control: Colon: An at least moderate collagen IV immunostaining of basement membranes and a strong staining of vessels and the tunica muscularis mucosa should be seen.
Negative Control: Colon: Collagen IV immunostaining must be absent in epithelial cells.
- 实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- 抗原
- Collagen IV (COL4) (Collagen, Type IV (COL4))
- 别名
- Collagen IV (COL4 产品)
- 别名
- collagen, type IV antibody, Let-2 antibody
- 背景
- Arresten, BSVD, COL4A1, COL4A1 NC1 domain, COL4A2, COL4A3, COL4A4, COL4A5, collagen alpha-1(IV) chain, Collagen IV Alpha 1 Polypeptide, Collagen IV Alpha 2 Polypeptide, Collagen Type IV Alpha 2, Collagen Type IV Alpha 3, Collagen Type IV Alpha 4, Collagen Type IV Alpha 5, RATOR,Collagen IV antibody validated for immunohistochemistry on 76 different Normal Tissues
- UniProt
- P02462, P08572, P29400, P53420
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