CD163 抗体 (AA 43-196)
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- 抗原 See all CD163 抗体
- CD163
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抗原表位
- AA 43-196
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD163 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC)
- 免疫原
- Recombinant fragment of human CD163 protein (around aa 43-196) (exact sequence is proprietary)
- 克隆位点
- MSVA-163M
- 亚型
- IgG
- Top Product
- Discover our top product CD163 Primary Antibody
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- 应用备注
- IHC 1:100-1:200
- 说明
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Positive Control: A moderate to strong cytoplasmic staining of macrophages should be seen in the interfollicular zones of the tonsil, lamina propria of the appendix and in the Kupffer cells of the liver (see images). Macrophages surrounding the vessels in brain specimens should stain positive, while microglial cells should not show CD163 staining.
Negative Control: CD163 staining should not be seen in hepatocytes and in epithelial cells of the appendix and tonsil.
- 实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- 抗原
- CD163
- 别名
- CD163 (CD163 产品)
- 别名
- DMBT1 antibody, CD163 antibody, CD163v2 antibody, CD163v3 antibody, M130 antibody, MM130 antibody, deleted in malignant brain tumors 1 protein-like antibody, CD163 molecule antibody, CD163 antigen antibody, LOC426820 antibody, CD163 antibody, Cd163 antibody
- 背景
- CD163, CD163 antigen, Macrophage-associated antigen, M130, CD163 Molecule, Hemoglobin scavenger receptor, MM130, Scavenger receptor cysteine rich type 1 protein M130,CD163 antibody validated for immunohistochemistry on 76 different Normal Tissues
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