CD27 抗体 (AA 28-170)
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- 抗原 See all CD27 抗体
- CD27 (CD27 Molecule (CD27))
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抗原表位
- AA 28-170
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD27 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC)
- 免疫原
- Recombinant human CD27 protein fragment (aa 28-170) (exact sequence is proprietary)
- 克隆位点
- MSVA-027M
- 亚型
- IgG
- Top Product
- Discover our top product CD27 Primary Antibody
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- 应用备注
- IHC 1:100-1:200
- 说明
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Positive Control: Tonsil: A strong, membranous staining should be seen in the majority of interfollicular lymphocytes but also in a fraction of lymphocytes in the germinal centres and the mantle zone.
Negative Control: Tonsil: CD27 staining should be absent in all epithelial cells and a fraction of lymphocytes.
- 实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- 抗原
- CD27 (CD27 Molecule (CD27))
- 别名
- CD27 (CD27 产品)
- 别名
- TNFRSF7 antibody, S152 antibody, Tnfrsf7 antibody, Tp55 antibody, S152. LPFS2 antibody, T14 antibody, CD27 molecule antibody, CD27 antigen antibody, CD27 antibody, Cd27 antibody
- 背景
- LPFS2, S152, T cell activation antigen S152, T-cell activation antigen CD27, T14, TNFRSF7, TNFSF7, Tp55, Tumor necrosis factor receptor superfamily member 7,CD27 antibody validated for immunohistochemistry on 76 different Normal Tissues
- UniProt
- P26842
- 途径
- Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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