Recombinant CD22 抗体 (AA 52-178)
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- 抗原 See all CD22 抗体
- CD22 (CD22 Molecule (CD22))
- 抗体类型
- Recombinant Antibody
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抗原表位
- AA 52-178
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适用
- 人
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宿主
- 兔
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克隆类型
- 单克隆
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标记
- This CD22 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC)
- 免疫原
- Recombinant fragment (around aa52-178) of human BL-CAM/CD22 protein (exact sequence is proprietary)
- 克隆位点
- MSVA-022R
- 亚型
- IgG
- Top Product
- Discover our top product CD22 Primary Antibody
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- 应用备注
- IHC 1:100-1:200
- 说明
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Positive Control: Tonsil or appendix: A strong, predominantly membranous CD22 staining of the germinal centre and mantle zone B-cells as well as of few interfollicular B-cells should be seen.
Negative Control: Tonsil or appendix: A large fraction of the interfollicular lymphocytes and all epithelial cells must not show any CD22 staining.
- 实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- 抗原
- CD22 (CD22 Molecule (CD22))
- 别名
- CD22 (CD22 产品)
- 别名
- A530093D23 antibody, Lyb-8 antibody, Lyb8 antibody, SIGLEC-2 antibody, SIGLEC2 antibody, siglec-2 antibody, CD22 antigen antibody, CD22 molecule antibody, Cd22 antibody, CD22 antibody
- 背景
- B-lymphocyte cell adhesion molecule (BL-CAM), B-cell receptor CD22, CD22, Lectin 2, Lyb8, Sialic acid-binding Ig-like lectin 2 (Siglec-2), SIGLEC2, T-cell surface antigen Leu-14,CD22 antibody validated for immunohistochemistry on 76 different Normal Tissues
- UniProt
- P20273
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