Recombinant CD5 抗体 (AA 32-372)
-
- 抗原 See all CD5 抗体
- CD5
- 抗体类型
- Recombinant Antibody
-
抗原表位
- AA 32-372
-
适用
- 人
-
宿主
- 兔
-
克隆类型
- 单克隆
-
标记
- This CD5 antibody is un-conjugated
-
应用范围
- Immunohistochemistry (IHC)
- 免疫原
- Recombinant fragment (around aa32-372) corresponding to human CD5 protein (extracellular).
- 克隆位点
- MSVA-005R
- 亚型
- IgG
- Top Product
- Discover our top product CD5 Primary Antibody
-
-
- 应用备注
- IHC 1:100-1:200
- 说明
-
Positive Control: Tonsil: A strong CD5 staining of virtually all T-cells in both T-zones and in germinal centres as well as an at least weak to moderate staining of dispersed B-cells in the mantle zone should be seen.
Negative Control: Tonsil: CD5 staining should be absent in germinal centre B-cells and in surface epithelial cells (A fraction of crypt epithelial cells may stain positive).
- 实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
-
- 抗原
- CD5
- 别名
- CD5 (CD5 产品)
- 别名
- CD5 antibody, Ly-1 antibody, Ly-12 antibody, Ly-A antibody, Lyt-1 antibody, LEU1 antibody, T1 antibody, CD5 molecule antibody, CD5 antigen antibody, CD5 molecule like antibody, Cd5 molecule antibody, CD5 antibody, Cd5 antibody, CD5L antibody
- 背景
- CD5 antigen (p56 62), LEU1, Ly12, LyA, Lymphocyte antigen T1/Leu-1, Lymphocyte glycoprotein T1/Leu1, T-cell surface glycoprotein CD5,CD5 antibody validated for immunohistochemistry on 76 different Normal Tissues
- UniProt
- P06127
-