gamma H2AX 抗体 (Ser140)
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- 抗原 See all gamma H2AX (GAMMA-H2AX) products
- gamma H2AX (GAMMA-H2AX) (gamma Histone Variant H2AX (GAMMA-H2AX))
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抗原表位
- Ser140
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This gamma H2AX antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (fixed cells) (IF/ICC)
- 原理
- Rabbit anti-gamma-H2AX Antibody, Affinity Purified
- 纯化方法
- Affinity Purified
- 免疫原
- surrounding serine 140
- 亚型
- IgG
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- 应用备注
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ICC-IF: 1:500 - 1:5,000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
IHC: 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
WB: 1:10,000 - 1:25,000
- 限制
- 仅限研究用
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- 浓度
- 1000 μg/mL
- 缓冲液
- Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 有效期
- 12 months
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- 抗原
- gamma H2AX (GAMMA-H2AX) (gamma Histone Variant H2AX (GAMMA-H2AX))
- 别名
- gamma-H2AX (GAMMA-H2AX 产品)
- 背景
- Background: H2AX is a member of the histone H2A family. The four core histones involved in the formation of the nucleosome structure of compacted chromatin are H2A, H2B, H3, and H4. H2AX may function to facilitate DNA repair, and recent studies have shown that H2AX is required for the maintenance of genomic stability. Gamma H2AX is the phosphorylated form of H2AX that results in response to DNA damage.
- 基因ID
- 3014
- NCBI登录号
- NP_002096
- UniProt
- P16104
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