Rad6 (N-Term) 抗体
-
- 抗原
- Rad6
-
抗原表位
- N-Term
-
适用
- 人, 小鼠
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- 非结合性
-
应用范围
- Western Blotting (WB), Immunohistochemistry (IHC)
- 原理
- Rabbit anti-Rad6 Antibody, Affinity Purified
- 预测反应
- Rat,D. melanogaster,Bovine,Rabbit
- 纯化方法
- Affinity Purified
- 免疫原
- N-terminus
- 亚型
- IgG
-
-
- 应用备注
-
IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
WB: 1:10,000 - 1:25,000
- 限制
- 仅限研究用
-
- 浓度
- 1000 μg/mL
- 缓冲液
- Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 有效期
- 12 months
-
- 抗原
- Rad6
- 背景
- Background: Rad 6, also known as Ubiquitin conjugating enzyme E2A, is an E2 enzyme of the three step ubiquitination pathway. Targeting of proteins by ubiquitination occurs in three main steps that involve the activation of ubiquitin by E1 enzymes, the carrying of ubiquitin to the target protein by E2 enzymes, and the ligation of ubiquitin to the target protein by E3 enzymes. Ubiquitination provides an important post-translational means to regulate protein abundance and activity. Rad6 has been demonstrated to be important to DNA damage repair. Mutations in Rad6 cause an X-linked mental retardation syndrome.
- 基因ID
- 7319
- NCBI登录号
- NP_003327
- UniProt
- P49459
-