ZEBOV GP 抗体
ZEBOV GP
适用: Zaire ebolavirus
ELISA, WB
宿主: 兔
Polyclonal
unconjugated
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- 抗原 See all ZEBOV GP products
- ZEBOV GP (Zaire Ebola Virus Envelope Glycoprotein (ZEBOV GP))
- 适用
- Zaire ebolavirus
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ZEBOV GP antibody is un-conjugated
- 应用范围
- ELISA, Western Blotting (WB)
- 特异性
- Anti-Ebola virus EBOV(subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) Glycoprotein/GP Polyclonal Antibody
- 纯化方法
- Antigen Affinity
- 免疫原
- Recombinant EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) Glycoprotein / GP Protein (His Tag), ABIN7198910
- 亚型
- IgG
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- 应用备注
- WB 1:1000-1:5000 ELISA 1:5000-1:10000
- 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- 0.2 μm filtered solution in PBS
- 储存条件
- -20 °C
- 储存方法
- Store at -20°C. Avoid freeze / thaw cycles.
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- 抗原
- ZEBOV GP (Zaire Ebola Virus Envelope Glycoprotein (ZEBOV GP))
- 别名
- ZEBOV Glycoprotein/GP (ZEBOV GP 产品)
- 背景
- Glycoprotein,GP,The fourth gene of the EBOV genome encodes a 16- kDa envelope-attached glycoprotein (GP) and a 11 kDa secreted glycoprotein (sGP). Both GP and sGP have an identical 295-residue N-terminus, however, they have different C-terminal sequences. Recently, great attention has been paid to GP for vaccines design and entry inhibitors isolation. GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . The GP1 subunit contains a mucin domain and a receptor-binding domain (RBD), the GP2 subunit has a fusion peptide, a helical heptad-repeat (HR) region, a transmembrane (TM) domain, and a 4-residue cytoplasmic tail. The RBD of GP1 mediates the interaction of EBOV with cellular receptor (e.g. DC-SIGN/LSIGN, TIM-1, hMGL, NPC1, β-integrins, folate receptor-α, and Tyro3 family receptors), of which TIM1 and NPC1 are essential for EBOV entry, the mucin domain having N- and O-linked glycans enhances the viral attachment to cellular hMGL, and participates in shielding key neutralization epitopes, which helps the virus evades immune elimination. There are large conformation changes of GP2 during membrane fusion, which enhance the insertion of fusion loop into cellular membrane and facilitate the release of viral nucleocapsid core to cytoplasm.
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