Recombinant mRuby 抗体 (DBCO)
-
- 抗原
- mRuby
- 抗体类型
- Recombinant Antibody
- 片段
- single-domain Antibody (sdAb)
-
适用
- Entacmaea quadricolor
-
宿主
-
Alpaca
- Expression System
- E.coli
-
克隆类型
- 单克隆
-
标记
- DBCO
-
应用范围
- Immunofluorescence (IF), Immunocytochemistry (ICC)
- 特异性
- Recognizes most common mRuby derivatives
- 纯化方法
- Produced in: E.coli
- 免疫原
- sdAb anti-mRUBY is derived from an in-house developed single-domain antibody (sdAb) that recognizes mRuby and mRuby derivatives, which is a bright monomeric red fluorescent protein derived from Entacmaea quadricolor.
- 克隆位点
- 1F8
-
anti-mRuby antibody
适用: Discosoma IF, IEM, IHC (fro), IHC (p), WB 宿主: 山羊 Polyclonal unconjugated
anti-mRuby antibody (Atto 488)适用: Entacmaea quadricolor IF, ICC, FACS 宿主: Alpaca Monoclonal 1F8 Atto 488 single-domain Antibody (sdAb) Recombinant Antibody
-
- 应用备注
- Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Reconsitute with 250 µL 50% glycerol for storage at -80 °C
- 缓冲液
- sdAbs are lyophilized from PBS pH 7.4.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.
-
- 抗原
- mRuby
- 背景
- MRuby is a bright monomeric red fluorescent protein, which has more than 20 mutations compared to its wild-type predecessor (eqFP611) found in Entacmaea quadricolor . mRuby, mRuby2 & mRuby3 are all exited at 560 nm, with mRuby3 being the brightest.
-
