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EAF1 抗体 (AA 50-120)

EAF1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN7266919
发货至: 中国
  • 抗原 See all EAF1 抗体
    EAF1 (ELL Associated Factor 1 (EAF1))
    抗原表位
    • 7
    • 6
    • 6
    • 6
    • 3
    • 1
    AA 50-120
    适用
    • 19
    • 1
    • 1
    宿主
    • 15
    • 4
    克隆类型
    • 17
    • 2
    多克隆
    标记
    • 9
    • 2
    • 2
    • 2
    • 2
    • 2
    This EAF1 antibody is un-conjugated
    应用范围
    • 19
    • 16
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB)
    原理
    EAF1 Rabbit pAb
    序列
    LQVGKGDEVT ITLPHIPGST PPMTVFKGNK RPYQKDCVLI INHDTGEYVL EKLSSSIQVK KTRAEGSSKI Q
    交叉反应
    人, 大鼠
    产品特性
    Polyclonal Antibodies
    纯化方法
    Affinity purification
    免疫原
    Recombinant fusion protein containing a sequence corresponding to amino acids 50-120 of human EAF1 (NP_149074.3).
    亚型
    IgG
    Top Product
    Discover our top product EAF1 Primary Antibody
  • 应用备注
    WB,1:500 - 1:2000
    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    Store at -20°C. Avoid freeze / thaw cycles.
  • 抗原
    EAF1 (ELL Associated Factor 1 (EAF1))
    别名
    EAF1 (EAF1 产品)
    别名
    4933403C17Rik antibody, AI666536 antibody, ELL associated factor 1 antibody, EAF1 antibody, Eaf1 antibody
    背景
    Actin is a key regulator of RNA polymerase (Pol) II-dependent transcription. Positive transcription elongation factor b (P-TEFb), a Cdk9/cyclin T1 heterodimer, has been reported to play a critical role in transcription elongation. However, the relationship between actin and P-TEFb is still not clear. In this study, actin was found to interact with Cdk9, a catalytic subunit of P-TEFb, in elongation complexes. Using immunofluorescence and immunoprecipitation assays, Cdk9 was found to bind to G-actin through the conserved Thr-186 in the T-loop. Overexpression and in vitro kinase assays showed that G-actin promotes P-TEFb-dependent phosphorylation of the Pol II C-terminal domain. An in vitro transcription experiment revealed that the interaction between G-actin and Cdk9 stimulated Pol II transcription elongation. ChIP and immobilized template assays indicated that actin recruited Cdk9 to a transcriptional template in vivo and in vitro. Using cytokine IL-6-inducible p21 gene expression system, we revealed that actin recruited Cdk9 to endogenous gene. Moreover, overexpression of actin and Cdk9 increased histone H3 acetylation and acetylized histone H3 binding to a transcriptional template through the interaction with histone acetyltransferase, p300. Taken together, our results suggested that actin participates in transcription elongation by recruiting Cdk9 for phosphorylation of the Pol II C-terminal domain, and the actin-Cdk9 interaction promotes chromatin remodeling.,EAF1,Epigenetics & Nuclear Signaling,EAF1
    分子量
    17kDa/29kDa
    基因ID
    85403
    UniProt
    Q96JC9
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