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- 抗原 See all EAF1 抗体
- EAF1 (ELL Associated Factor 1 (EAF1))
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抗原表位
- AA 50-120
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This EAF1 antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 原理
- EAF1 Rabbit pAb
- 序列
- LQVGKGDEVT ITLPHIPGST PPMTVFKGNK RPYQKDCVLI INHDTGEYVL EKLSSSIQVK KTRAEGSSKI Q
- 交叉反应
- 人, 大鼠
- 产品特性
- Polyclonal Antibodies
- 纯化方法
- Affinity purification
- 免疫原
- Recombinant fusion protein containing a sequence corresponding to amino acids 50-120 of human EAF1 (NP_149074.3).
- 亚型
- IgG
- Top Product
- Discover our top product EAF1 Primary Antibody
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anti-ELL Associated Factor 1 (EAF1) (AA 1-268) antibody
EAF1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1G2 unconjugated
anti-ELL Associated Factor 1 (EAF1) (AA 1-268) antibodyEAF1 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
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- 应用备注
- WB,1:500 - 1:2000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20°C. Avoid freeze / thaw cycles.
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- 抗原
- EAF1 (ELL Associated Factor 1 (EAF1))
- 别名
- EAF1 (EAF1 产品)
- 别名
- 4933403C17Rik antibody, AI666536 antibody, ELL associated factor 1 antibody, EAF1 antibody, Eaf1 antibody
- 背景
- Actin is a key regulator of RNA polymerase (Pol) II-dependent transcription. Positive transcription elongation factor b (P-TEFb), a Cdk9/cyclin T1 heterodimer, has been reported to play a critical role in transcription elongation. However, the relationship between actin and P-TEFb is still not clear. In this study, actin was found to interact with Cdk9, a catalytic subunit of P-TEFb, in elongation complexes. Using immunofluorescence and immunoprecipitation assays, Cdk9 was found to bind to G-actin through the conserved Thr-186 in the T-loop. Overexpression and in vitro kinase assays showed that G-actin promotes P-TEFb-dependent phosphorylation of the Pol II C-terminal domain. An in vitro transcription experiment revealed that the interaction between G-actin and Cdk9 stimulated Pol II transcription elongation. ChIP and immobilized template assays indicated that actin recruited Cdk9 to a transcriptional template in vivo and in vitro. Using cytokine IL-6-inducible p21 gene expression system, we revealed that actin recruited Cdk9 to endogenous gene. Moreover, overexpression of actin and Cdk9 increased histone H3 acetylation and acetylized histone H3 binding to a transcriptional template through the interaction with histone acetyltransferase, p300. Taken together, our results suggested that actin participates in transcription elongation by recruiting Cdk9 for phosphorylation of the Pol II C-terminal domain, and the actin-Cdk9 interaction promotes chromatin remodeling.,EAF1,Epigenetics & Nuclear Signaling,EAF1
- 分子量
- 17kDa/29kDa
- 基因ID
- 85403
- UniProt
- Q96JC9
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