Immunofluorescence (fixed cells) (IF/ICC), Immunohistochemistry (IHC), Western Blotting (WB)
原理
Anti-Neuroligin-4 Mouse Monoclonal Antibody
特异性
This antibody recognizes mouse Neuroligin-4. It does not cross-react with other Neuroligins.
交叉反应
小鼠, 大鼠
免疫原
Fusion protein corresponding to aa 782-945 (intracellular C-terminus) of mouse Neuroligin-4.
克隆位点
S98-7
亚型
IgG1
应用备注
Immunoblotting: use at 1-2 μg/mL. A band of ~125-130 kDa is detected. Immunofluorescence: use at 10 μg/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their application.
限制
仅限研究用
状态
Liquid
溶解方式
Dilute in PBS or medium that is identical to that used in the assay system.
浓度
1.0 mg/mL
缓冲液
PBS, pH 7.4, 0.1 % sodium azide, 50 % glycerol.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
This product is stable for at least one (1) year at -20°C.
Neuroligin 4-like,Cell surface protein involved in cell-cell-interactions. Plays a role in the formation or maintenance of synaptic junctions via its interactions (via the extracellular domains) with neurexin family members. Plays a role in synaptic signal transmission. {PubMed:18434543, PubMed:21282647}.,Neuroligin-4 is a neuronal cell surface protein and a member of the type-B carboxylesterase- lipase protein family. Members of this family may act as splice site-specific ligands for beta- neurexins and may be involved in the formation and remodeling of central nervous system synapses. Neuroligin-4 interacts with discs large homolog 4 (DLG4). Mutations in this gene have been associated with autism and Asperger syndrome. Alternative splicing results in multiple transcript variants.,Cell membrane, Single-pass type I membrane protein. Cell junction, synapse, postsynaptic cell membrane. Note=Detected at glycinergic postsynapses in retina. Detected on dendritic spines on cultured neurons.,Neuroligin-4, NL-4