PI3K p85 alpha/gamma 抗体
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- 抗原 See all PI3K p85 alpha/gamma (PI3K p85a/g) products
- PI3K p85 alpha/gamma (PI3K p85a/g) (Phosphoinositide 3 Kinase, p85 alpha/gamma (PI3K p85a/g))
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适用
- 人, 小鼠, 大鼠, 猴, 小鸡
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PI3K p85 alpha/gamma antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 原理
- PI 3-kinase p85α/γ Polyclonal Antibody
- 特异性
- PI 3-kinase p85α/γ Polyclonal Antibody detects endogenous levels of PI 3-kinase p85α/γ protein.
- 纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
- 免疫原
- Synthesized peptide derived from human PI 3-kinase p85/p55 around the non-phosphorylation site of Y458/199
- 亚型
- IgG
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- 应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.
- 说明
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Primary Antibody
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- 抗原
- PI3K p85 alpha/gamma (PI3K p85a/g) (Phosphoinositide 3 Kinase, p85 alpha/gamma (PI3K p85a/g))
- 别名
- PI 3-kinase p85alpha/gamma (PI3K p85a/g 产品)
- 背景
- Rabbit Anti-PI 3-kinase p85α/γ Polyclonal Antibody,PIK3R1, GRB1, Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alph,Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. PIK3R1 encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in PIK3R1 has been associated with insulin resistance. Alternative splicing of PIK3R1 results in four transcript variants encoding different isoforms.,Phosphatidylinositol 3-kinase regulatory subunit alpha
- 分子量
- observerd band 54+83kDa
- 基因ID
- 5295, 8503
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