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Vimentin 抗体 (AA 2-466)

Name: Vimentin 抗体 (AA 2-466)
Brand: antibodies-online
SKU: ABIN7193582
Availability: OutOfStock

VIM 适用: 人 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 3A1F2 unconjugated

Western blot analysis using VIM mAb against HEK293 (1) and VIM (AA: 2-466)-hIgGFc transfected HEK293 (2) cell lysate. (Vimentin 抗体 (AA 2-466))

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using VIM mouse mAb with DAB staining. (Vimentin 抗体 (AA 2-466))

IHC

Black line: Control Antigen (100 ng),Purple line: Antigen (10 ng), Blue line: Antigen (50 ng), Red line:Antigen (100 ng) (Vimentin 抗体 (AA 2-466))

ELISA

Western blot analysis using VIM mAb against human VIM (AA: 2-466) recombinant protein. (Vimentin 抗体 (AA 2-466))

Western blot analysis using VIM mouse mAb against Jurkat (1), K562 (2), SK-N-SH (3), SH-SY5Y (4), Hela (5), NIH/3T3 (6), C6 (7), and RAW264. (Vimentin 抗体 (AA 2-466))

Flow cytometric analysis of Hela cells using VIM mouse mAb (green) and negative control (red). (Vimentin 抗体 (AA 2-466))

FACS

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using VIM mouse mAb with DAB staining. (Vimentin 抗体 (AA 2-466))

IHC

7 images

产品编号 ABIN7193582

发货至: 中国

Datasheet as PDF

抗原 See all Vimentin (VIM) 抗体

Vimentin (VIM)
抗原表位
32

16

15

15

11

9

9

7

4

3

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 2-466
适用
304

120

117

60

60

49

42

37

29

21

20

13

13

8

8

6

6

3

3

2

1

1

1

1

1

1

1

1

1
人
宿主
158

129

11

6

6

5

2
小鼠
Compatible Secondaries
克隆类型
172

144
单克隆
标记
197

15

13

12

9

7

3

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1
This Vimentin antibody is un-conjugated
应用范围
262

114

103

97

87

64

48

40

39

33

23

20

8

5

4

4

3

2

2

2

1

1

1

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

原理

Vimentin Antibody

纯化方法

Purified antibody

免疫原

Purified recombinant fragment of human VIM (AA: 2-466) expressed in E. Coli.

克隆位点

3A1F2

亚型

IgG2a

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VIM 适用: 人, 小鼠, 大鼠, Pig, 小鸡 WB, IHC (p), ICC, IP 宿主: 小鼠 Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal C8 unconjugated

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VIM 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 3E9 unconjugated

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VIM 适用: 人, Pig, Cow, 小鸡, 犬, Cat, 山羊 WB, IHC, FACS, StM 宿主: 小鼠 Monoclonal VM452 unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人 WB, IHC, StM 宿主: 兔 Monoclonal VIM-1937R unconjugated Recombinant Antibody

anti-Vimentin (VIM) (AA 210-466) antibody
VIM 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 5D7 unconjugated

应用备注

ELISA: 1/10000
FCM: 1/200-1/400

限制

仅限研究用
状态

Liquid

缓冲液

Purified antibody in PBS with 0.05 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
抗原

Vimentin (VIM)

别名

Vimentin (VIM 产品)

背景

Description:
This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients.
Aliases: FLJ36605, VIM

分子量

53.7kDa

基因ID

7431

UniProt

P08670

途径

Caspase Cascade in Apoptosis