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- 抗原
- S1A9
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抗原表位
- AA 2-113
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适用
- 小鼠
-
宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- ELISA, Western Blotting (WB), Immunoprecipitation (IP)
- 交叉反应
- 小鼠
- 纯化方法
- >95%, Protein G purified
- 免疫原
- Recombinant Mouse Protein S100-A9 protein (2-113AA)
- 亚型
- IgG
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anti-S1A9 antibody
适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 1G1C1 unconjugated
anti-S1A9 (AA 2-114) antibody适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-S1A9 antibody适用: 人 ELISA, IHC 宿主: 兔 Monoclonal 2E9 unconjugated Recombinant Antibody
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- 应用备注
- Recommended dilution: WB:1:500-1:5000, IP:1:200-1:2000,
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
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Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4 - 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
-
- 抗原
- S1A9
- 背景
-
Background: S100A9 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis, adhesion, can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2 and can induce degranulation of neutrophils by a MAPK-dependent mechanism. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinfammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn2+ which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect, regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants.
Aliases: S100a9 antibody, Cagb antibody, Mrp14 antibody, Protein S100-A9 antibody, Calgranulin-B antibody, Leukocyte L1 complex heavy chain antibody, Migration inhibitory factor-related protein 14 antibody, MRP-14 antibody, p14 antibody, S100 calcium-binding protein A9 antibody
- UniProt
- P31725
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