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ATG3 抗体 (AA 1-314)

Name: ATG3 抗体 (AA 1-314)
Brand: antibodies-online
SKU: ABIN7174567
Availability: OutOfStock

ATG3 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

Western Blot Positive WB detected in: K562 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, PC-3 whole cell lysate All lanes: ATG3 antibody at 4 μg/mL Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa (ATG3 抗体 (AA 1-314))

IHC image of ABIN7174567 diluted at 1:1200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. (ATG3 抗体 (AA 1-314))

IHC

Immunofluorescence staining of Hela cells with ABIN7174567 at 1:400, counter-stained with DAPI. (ATG3 抗体 (AA 1-314))

IHC image of ABIN7174567 diluted at 1:1200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. (ATG3 抗体 (AA 1-314))

IHC

4 images

产品编号 ABIN7174567

发货至: 中国

Datasheet as PDF

抗原 See all ATG3 抗体

ATG3 (ATG3 Autophagy Related 3 (ATG3))
抗原表位
22

15

14

11

6

6

4

4

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1
AA 1-314
适用
105

42

41

7

5

4

4

3

3

2

1

1

1

1

1
人
宿主
106

12

3
兔
Compatible Secondaries
克隆类型
102

19
多克隆
标记
40

11

11

10

7

6

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1
This ATG3 antibody is un-conjugated
应用范围
78

53

26

26

17

15

11

10

6

6

5

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

交叉反应

人

纯化方法

>95%, Protein G purified

免疫原

Recombinant Human Ubiquitin-like-conjugating enzyme ATG3 protein (1-314AA)

亚型

IgG

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anti-ATG3 Autophagy Related 3 (ATG3) antibody (PerCP)
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anti-ATG3 Autophagy Related 3 (ATG3) antibody (APC)
ATG3 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal APC

anti-ATG3 Autophagy Related 3 (ATG3) (AA 1-100) antibody
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anti-ATG3 Autophagy Related 3 (ATG3) antibody (HRP)
ATG3 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal HRP

anti-ATG3 Autophagy Related 3 (ATG3) antibody (Alkaline Phosphatase (AP))
ATG3 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal Alkaline Phosphatase (AP)

应用备注

Recommended dilution: WB:1:500-1:5000, IHC:1:1000-1:2000, IF:1:200-1:500,

限制

仅限研究用
状态

Liquid

缓冲液

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C,-80 °C

储存方法

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
抗原

ATG3 (ATG3 Autophagy Related 3 (ATG3))

别名

ATG3 (ATG3 产品)

别名

atg3 antibody, im:6910145 antibody, wu:fb15g12 antibody, zgc:64156 antibody, APG3 antibody, APG3-LIKE antibody, APG3L antibody, PC3-96 antibody, 2610016C12Rik antibody, Apg3l antibody, Atg3l antibody, PIG-1 antibody, Pig1 antibody, ATATG3 antibody, K11J9.1 antibody, K11J9_1 antibody, autophagy related 3 antibody, autophagy 3 (APG3) antibody, similar to S. cerevisiae ATG3 (YNR007C) part of ubiquitin-like system that mediates Atg8-phosphatidylethanolamine conjugation required for protein-vacuolar targeting and autophagy antibody, atg3 antibody, ATG3 antibody, Atg3 antibody

背景

Background: E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.
Aliases: 2610016C12Rik antibody, Apg 3 antibody, APG3 antibody, APG3 autophagy 3 like antibody, APG3 like antibody, APG3, S. cerevisiae, homolog of antibody, APG3-like antibody, APG3L antibody, Apg3p antibody, ATG 3 antibody, ATG3 antibody, ATG3 autophagy related 3 homolog antibody, ATG3 autophagy related 3 homolog (S. cerevisiae) antibody, ATG3_HUMAN antibody, Autophagy 3, S. cerevisiae, homolog of antibody, Autophagy Apg3p/Aut1p like antibody, autophagy related 3 antibody, Autophagy related protein 3 antibody, Autophagy-related protein 3 antibody, DKFZp564M1178 antibody, FLJ22125 antibody, hApg3 antibody, MGC15201 antibody, OTTHUMP00000214547 antibody, OTTHUMP00000214548 antibody, PC3 96 antibody, Protein PC3-96 antibody, Ubiquitin-like-conjugating enzyme ATG3 antibody

UniProt

Q9NT62

途径

Autophagy