Retinoblastoma 1 抗体 (pSer795)
(1 validation)-
- 抗原 See all Retinoblastoma 1 (RB1) 抗体
- Retinoblastoma 1 (RB1)
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抗原表位
- pSer795
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Retinoblastoma 1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 预测反应
- Human,Mouse,Rat,Dog,Cow,Horse,Rabbit
- 纯化方法
- Purified by Protein A.
- 免疫原
- KLH conjugated synthetic phosphopeptide derived from human P105 RB around the phosphorylation site of Ser795
- 亚型
- IgG
- Top Product
- Discover our top product RB1 Primary Antibody
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anti-Retinoblastoma 1 (RB1) (C-Term) antibody
RB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Retinoblastoma 1 (RB1) (pSer795) antibodyRB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Retinoblastoma 1 (RB1) (pSer807) antibodyRB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Retinoblastoma 1 (RB1) (pSer780) antibodyRB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Retinoblastoma 1 (RB1) (C-Term) antibodyRB1 适用: 人 WB, IF, IP, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Retinoblastoma 1 (RB1) (pSer780) antibodyRB1 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Retinoblastoma 1 (RB1) (pSer807), (pSer811) antibodyRB1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Retinoblastoma 1 (RB1) (AA 639-778) antibodyRB1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Retinoblastoma 1 (RB1) (pSer780) antibodyRB1 适用: 人 IHC, ELISA, IF, IP 宿主: 兔 Monoclonal 2E9 unconjugated Recombinant Antibody
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- 应用备注
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WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - 限制
- 仅限研究用
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- by
- Kinexus Bioinformatics Corporation
- No.
- #029821
- 日期
- 2014.09.21
- 抗原
- Lot Number
- 121026
- Method validated
- Western Blotting
- Positive Control
- MCF7 cells
- Negative Control
- HCT-116 cells - low
- Notes
- A band was observed in the positive control at the expected size (~100-105 kDa), which was absent from the negative control. There were some additional bands observed at non-expected molecular weights in the positive control.
- Primary Antibody
- Antigen: Retinoblastoma 1 (RB1) (pSer795)
- Catalog number: ABIN712946
- Lot number: 121026
- Dilution: 1:100
- Secondary Antibody
- Antibody: Donkey anti-Rabbit IgG Antibody (HRP)
- Lot number: F0613
- Dilution: 1:10,000
- Full Protocol
- 1. Cell/tissue total protein lysates were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol at 95°C for 5 minutes prior to loading.
- 2. 15 μg of boiled lysate were loaded and resolved on a 12% SDS-polyacrylamide gel.
- 3. The Precision Plus Protein™ All Blue Prestained Standards from BioRad (161-0373) were used as molecular mass markers.
- 4. Proteins were transferred onto nitrocellulose membrane by tank transfer and protein transfer was confirmed with Ponceau S staining.
- 5. The immunoblot membrane was blocked in 2.5% skim milk and 1.5% BSA solution in TTBS at room temperature for 60 minutes.
- 6. The membrane was washed in TTBS twice for 5 minutes each.
- 7. The membrane was immersed with the protein side up in the antibody solution in TBS and incubated overnight at 4°C with gentle agitation.
- 8. The membrane was rinsed twice with TTBS.
- 9. The membrane was washed in TTBS twice for 5 minutes each.
- 10. The membrane was washed in TTBS once for 15 minutes.
- 11. The membrane was incubated in the HRP-conjugated secondary antibody solution in TBS for 60 minutes at room temperature with gentle agitation.
- 12. The membrane was rinsed twice with TTBS.
- 13. The membrane was washed in TTBS twice for 5 minutes each.
- 14. The membrane was washed in TTBS once for 15 minutes.
- 15. Signals were detected by chemiluminescence (ECL). The blot was scanned for 320 seconds.
- 16. The membrane was rinsed three times with TTBS.
- 17. Repeated Steps 4-15 with the loading control antibody and its matching secondary antibody. The blot was scanned for 160 seconds.
- Experimental Notes
- - No experimental challenges noted.
生效 #029821 (Western Blotting)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Figure 1: Western Blot for RB1 (pSer795). Grey arrowhead indicates the expected molecular weight of ~100-105 kDa.]()
Figure 1: Western Blot for RB1 (pSer795). Grey arrowhead indicates the expected molecular weight of ~100-105 kDa.
Full Methods -
- 状态
- Liquid
- 浓度
- 1 μg/μL
- 缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- 有效期
- 12 months
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- 抗原
- Retinoblastoma 1 (RB1)
- 别名
- P105 RB (RB1 产品)
- 别名
- OSRC antibody, RB antibody, p105-Rb antibody, pRb antibody, pp110 antibody, Rb antibody, Rb-1 antibody, P105-RB antibody, PP110 antibody, RB11 antibody, zgc:154147 antibody, RB transcriptional corepressor 1 antibody, RB transcriptional corepressor like 1 antibody, retinoblastoma 1 antibody, RB1 antibody, RBL1 antibody, Rb1 antibody, rb1 antibody
- 背景
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Synonyms: RbSer795, RB1phospho S795, OSRC, P105 RB, P105RB, PP105, PP110, pRb, RB 1, RB1, RB1 protein, Retinoblastoma 1 including osteosarcoma, Retinoblastoma 1, Retinoblastoma associated protein, Retinoblastoma related osteosarcoma, Retinoblastoma susceptibility gene, Including osteosarcoma, RB_HUMAN.
Background: Rb is a tumor suppressor gene which functions as a negative regulator of the cell cycle by interacting with transcription factors including E2F1, PU1, ATF2, UBF, Elf1 and cAbl. This ability of Rb to alter transcription is regulated by phosphorylation catalyzed by the cyclin dependent protein kinases (cdks). Rb is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus 16E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the Rb gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.
- 基因ID
- 5925
- 途径
- Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy
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