PKM 抗体
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- 抗原 See all PKM 抗体
- PKM (Pyruvate Kinase M1/2 (PKM))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This PKM antibody is un-conjugated
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应用范围
- ELISA, Western Blotting (WB), Immunoprecipitation (IP)
- 交叉反应
- 人
- 纯化方法
- Affinity purified
- 免疫原
- Recombinant human PKM2 protein
- 亚型
- IgG
- Top Product
- Discover our top product PKM Primary Antibody
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anti-Pyruvate Kinase M1/2 (PKM) (AA 2-531) antibody
PKM 适用: 人 ELISA, WB, IHC, IF, IP, FACS 宿主: 小鼠 Monoclonal 6C3C7 unconjugated
anti-Pyruvate Kinase M1/2 (PKM) (AA 11-221) antibodyPKM 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase M1/2 (PKM) antibodyPKM 适用: 人 ELISA, WB, IHC, IF, IP, FACS 宿主: 兔 Monoclonal 7B2 unconjugated Recombinant Antibody
anti-Pyruvate Kinase M1/2 (PKM) (AA 185-461) antibodyPKM 适用: 人 ELISA, WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase M1/2 (PKM) (Internal Region) antibodyPKM 适用: 人, 小鼠, 大鼠 ELISA, WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase M1/2 (PKM) (AA 2-192) antibodyPKM 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase M1/2 (PKM) (pSer37) antibodyPKM 适用: 人 ELISA, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase M1/2 (PKM) antibodyPKM 适用: 人, 小鼠, 大鼠 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase M1/2 (PKM) (pSer37) antibodyPKM 适用: 人, 小鼠, 大鼠 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase M1/2 (PKM) (pTyr105) antibodyPKM 适用: 人, 小鼠 ELISA, WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Recommended dilution:WB:1:500-1:5000,IP:1:200-1:2000,
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 1.0 mg/mL in phosphate buffer without Mg2+/Ca2+, pH 7.4, 150 mM NaCl and 50 % glycerol
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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- 抗原
- PKM (Pyruvate Kinase M1/2 (PKM))
- 别名
- PKM (PKM 产品)
- 别名
- CTHBP antibody, OIP3 antibody, PK3 antibody, PKM2 antibody, TCB antibody, THBP1 antibody, AA414905 antibody, AL024370 antibody, AL024424 antibody, Pk-2 antibody, Pk-3 antibody, Pk3 antibody, Pkm2 antibody, PKM12 antibody, CG7070 antibody, Dmel\\CG7070 antibody, PYK antibody, Pyk antibody, cb1029 antibody, fj33f03 antibody, pkm2 antibody, pkm2a antibody, wu:fj33f03 antibody, MGC53388 antibody, PKM antibody, PK-S antibody, pk3 antibody, pkm2b antibody, zgc:92037 antibody, pyruvate kinase M1/2 antibody, pyruvate kinase, muscle antibody, Pyruvate kinase antibody, pyruvate kinase M1/2a antibody, pyruvate kinase PKM antibody, pyruvate kinase (PK) antibody, pyruvate kinase antibody, pyruvate kinase M1/2 S homeolog antibody, pyruvate kinase M1/2b antibody, PKM antibody, Pkm antibody, PyK antibody, pkma antibody, pkm antibody, LOC710798 antibody, pyk antibody, pk antibody, LOC100534543 antibody, pkm.S antibody, pkmb antibody
- 背景
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Background: Pyruvate kinase (PK) regulates the final rate-limiting step of glycolysis in the production of pyruvate and adenosine triphosphate (ATP). Alternate splicing of PKM pre-mRNA leads to PKM2 generation by the inclusion of exon 10 and the exclusion of exon 9, which is specific for PKM1. Besides its cytosolic roles in glycolysis, PKM2, which is upregulated by growth factor receptor activation 1, is phosphorylated at S37 by extracellular signal-regulated kinase (ERK) 2. This phosphorylation leads to the cis-trans isomerization of PKM2 by the peptidyl-prolineisomerase protein interacting with never in mitosis A 1 (PIN1), exposure of the nuclear localization signal (NLS) of PKM2, and subsequent binding of importin α 5 for nuclear translocation 2. In the nucleus, PKM2binds to phosphorylated Y333 of β-catenin, which is essential for β-catenin transactivation 3, and interacts with and phosphorylates histone H3 at T11, leading to H3-K9 acetylation and transcription of genes such as MYC and CCND14. c-Myc expression results in the upregulation of GLUT1, lactate dehydrogenase A (LDHA), and, in a positive feedback loop, PTB-dependent PKM2, which subsequently enhances the Warburg effect 2. Cyclin D1 expression, in turn, promotes G1-S phase transition 3, 4.1 Yang W, Xia Y, Cao Y et al. EGFR-induced and PKCepsilonmonoubiquitylation-dependent NF-kappaB activation upregulates PKM2 expression and promotes tumorigenesis. Molecular cell 2012; 48:771-784.2 Yang W, Zheng Y, Xia Y et al. ERK1/2-dependent phosphorylation and nuclear translocation of PKM2 promotes the Warburg effect. Nature cell biology 2012; 14:1295-1304.3 Yang W, Xia Y, Ji H et al. Nuclear PKM2 regulates beta-catenin transactivation upon EGFR activation. Nature 2011; 480:118-122.4 Yang W, Xia Y, Hawke D et al. PKM2 phosphorylates histone H3 and promotes gene transcription and tumorigenesis. Cell 2012; 150:685-696.
Aliases: PKM; PK3; OIP3; PK2;
- UniProt
- P14618
- 途径
- Warburg Effect
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