Nanog 抗体 (AA 1-305)
-
- 抗原 See all Nanog (NANOG) 抗体
- Nanog (NANOG) (Nanog Homeobox (NANOG))
-
抗原表位
- AA 1-305
-
适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This Nanog antibody is un-conjugated
-
应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)
- 交叉反应
- 人, 小鼠, 大鼠
- 纯化方法
- Protein G purified
- 免疫原
- Recombinant Human Homeobox protein NANOG protein (1-305AA)
- 克隆位点
- 8A1D11
- 亚型
- IgG1, IgG1 kappa
- Top Product
- Discover our top product NANOG Primary Antibody
-
-
- 应用备注
- Recommended dilution: WB:1:500-1:2000, ICC:1:50-1:500, IF:1:50-1:200,
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
-
Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4 - 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
-
- 抗原
- Nanog (NANOG) (Nanog Homeobox (NANOG))
- 别名
- NANOG (NANOG 产品)
- 别名
- 2410002E02Rik antibody, ENK antibody, ecat4 antibody, NANOG antibody, hacp antibody, wu:fd19e04 antibody, wu:fd20a07 antibody, zgc:193933 antibody, Nanog homeobox antibody, nanog homeobox antibody, Nanog antibody, NANOG antibody, nanog antibody
- 背景
-
Background: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed:25825768). Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
Aliases: Homeobox protein NANOG (Homeobox transcription factor Nanog) (hNanog), NANOG
- UniProt
- Q9H9S0
- 途径
- Stem Cell Maintenance
-