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Hsc70 抗体 (AA 2-646)

Name: Hsc70 抗体 (AA 2-646)
Brand: antibodies-online
SKU: ABIN7154884
Availability: OutOfStock

HSPA8 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 小鼠 Monoclonal 6E4D2 unconjugated

Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate All lanes HSPA8 antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 70~75 KDa Observed band size: 70~75 KDa Exposure time: 5 min (Hsc70 抗体 (AA 2-646))

Overlay histogram showing MCF-7 cells stained with ABIN7154884 (red line). (Hsc70 抗体 (AA 2-646))

FACS

Western Blot Positive WB detected in: Hela whole cell lysate at 20 μg, 10 μg, 5 μg, 2. (Hsc70 抗体 (AA 2-646))

Western Blot Positive WB detected in: 20 μg hela whole cell lysate HSPA8 antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 70~75 KDa Observed band size: 70~75 KDa Exposure time: 5 min (Hsc70 抗体 (AA 2-646))

IHC image of ABIN7154884 diluted at 1:220 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. (Hsc70 抗体 (AA 2-646))

IHC

IHC image of ABIN7154884 diluted at 1:220 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. (Hsc70 抗体 (AA 2-646))

IHC

Immunofluorescence staining of Hela cells with ABIN7154884 at 1:100, counter-stained with DAPI. (Hsc70 抗体 (AA 2-646))

Immunofluorescence staining of MCF-7 cells with ABIN7154884 at 1:100, counter-stained with DAPI. (Hsc70 抗体 (AA 2-646))

Immunofluorescence staining of PC-3 cells with ABIN7154884 at 1:100, counter-stained with DAPI. (Hsc70 抗体 (AA 2-646))

9 images

产品编号 ABIN7154884

发货至: 中国

Datasheet as PDF

抗原 See all Hsc70 (HSPA8) 抗体

Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))
抗原表位
14

7

6

5

3

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 2-646
适用
81

48

44

21

17

14

13

11

11

11

10

10

5

5

5

4

4

3

3

3

2

1

1

1

1

1

1

1
人
宿主
70

27

7

2
小鼠
Compatible Secondaries
克隆类型
70

35
单克隆
标记
58

19

11

3

3

1

1

1

1

1

1

1

1

1

1

1

1
This Hsc70 antibody is un-conjugated
应用范围
92

51

39

29

29

27

15

13

11

11

11

5

4

2

2

2

2

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

交叉反应

人

纯化方法

Protein G purified

免疫原

Recombinant Human Heat shock cognate 71 kDa protein (2-646AA)

克隆位点

6E4D2

亚型

IgG1, IgG1 kappa
anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 2-646) antibody
HSPA8 适用: 人 WB, IHC, ELISA, IP, FACS 宿主: 小鼠 Monoclonal 2G8F6 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 1-300) antibody
HSPA8 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (C-Term) antibody
HSPA8 适用: 人, 大鼠, 小鼠 WB, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) antibody
HSPA8 适用: 人 WB, IHC, ELISA, IP, ICC, IF, PLA, Bind, AA 宿主: 小鼠 Monoclonal 1F2-H5 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 13-31), (N-Term) antibody
HSPA8 适用: 人, 大鼠, 小鼠 WB, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (N-Term) antibody
HSPA8 适用: 人, 大鼠, 小鼠, Cow, 绵羊, Pig, 斑马鱼, 山羊 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 534-615) antibody
HSPA8 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 418-597) antibody
HSPA8 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (Internal Region) antibody
Verified HSPA8 适用: 人, 小鼠 WB, IHC, ELISA, IF 宿主: 山羊 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) antibody (HRP)
HSPA8 适用: 人 WB, IHC, ELISA, IP, ICC, IF, PLA, Bind, AA 宿主: 小鼠 Monoclonal 1F2-H5 HRP

应用备注

Recommended dilution: WB: 1:5000-1:32000, IHC: 1:100-1:500, IF: 1:100-1:300, FC: 1:100-1:300,

限制

仅限研究用
状态

Liquid

缓冲液

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C,-80 °C

储存方法

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
抗原

Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

别名

HSPA8 (HSPA8 产品)

别名

hsc54 antibody, hsc70 antibody, hsc71 antibody, hsp71 antibody, hsp73 antibody, hspa10 antibody, lap1 antibody, nip71 antibody, HSC54 antibody, HSC70 antibody, HSC71 antibody, HSP71 antibody, HSP73 antibody, HSPA10 antibody, LAP1 antibody, NIP71 antibody, Hsc70 antibody, 2410008N15Rik antibody, Hsc71 antibody, Hsc73 antibody, Hsp73 antibody, Hspa10 antibody, wu:fb01g06 antibody, wu:fi48b06 antibody, heat shock protein family A (Hsp70) member 8 L homeolog antibody, heat shock protein family A (Hsp70) member 8 antibody, heat shock 70kDa protein 8 antibody, heat shock protein 8 antibody, hspa8.L antibody, HSPA8 antibody, Hspa8 antibody, hspa8 antibody

背景

Background: Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Participates in the ER-associated degradation (ERAD) quality control pathway in conjunction with J domain-containing co-chaperones and the E3 ligase CHIP
Aliases: Heat shock cognate 71 kDa protein (Heat shock 70 kDa protein 8) (Lipopolysaccharide-associated protein 1) (LAP-1) (LPS-associated protein 1), HSPA8, HSC70 HSP73 HSPA10

UniProt

P11142