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FZD7 抗体 (AA 501-574)

Name: FZD7 抗体 (AA 501-574)
SKU: ABIN710051
Availability: OutOfStock
Rating: 5 (1 reviews)

FZD7 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Western Blotting validation image for anti-Frizzled Family Receptor 7 (FZD7) (AA 501-574) antibody (ABIN710051) (FZD7 抗体 (AA 501-574))

Image provided by the Independent Validation Program (badge number 29805). (FZD7 抗体 (AA 501-574))

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Frizzled 7/FZE3 Polyclonal Antibody, Unconjugated (ABIN710051) at 1:200, followed by conjugation to the secondary antibody and DAB staining (FZD7 抗体 (AA 501-574))

IHC

Formalin-fixed and paraffin embedded human placenta tissue labeled with Anti-Frizzled 7/FZE3 Polyclonal Antibody, Unconjugated (ABIN710051) at 1:200, followed by conjugation to the secondary antibody and DAB staining (FZD7 抗体 (AA 501-574))

IHC

4 images

(1 reference)

(1 validation)

产品编号 ABIN710051

发货至: 中国

Datasheet as PDF

抗原 See all FZD7 抗体

FZD7 (Frizzled Family Receptor 7 (FZD7))
抗原表位
15

9

7

6

6

6

5

4

4

2

2

2

2

1

1

1

1

1

1
AA 501-574
适用
58

38

22

5

4

4

3

3

3

2

1

1

1

1
人, 小鼠, 大鼠
宿主
52

6

5

3
兔
Compatible Secondaries
克隆类型
55

10
多克隆
标记
33

7

5

4

3

2

1

1

1

1

1

1

1

1

1

1

1

1
This FZD7 antibody is un-conjugated
应用范围
55

37

17

12

12

11

7

6

2

2

2

1

1
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

人, 小鼠, 大鼠

预测反应

Dog,Cow,Pig,Chicken,Guinea Pig

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human Frizzled 7

亚型

IgG
anti-Frizzled Family Receptor 7 (FZD7) (AA 155-253) antibody
FZD7 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 4D9 unconjugated

anti-Frizzled Family Receptor 7 (FZD7) (C-Term) antibody
FZD7 适用: 人, 小鼠, 大鼠, 犬, Cow, 兔, Pig, 豚鼠, Saccharomyces cerevisiae WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Frizzled Family Receptor 7 (FZD7) (N-Term) antibody
FZD7 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Frizzled Family Receptor 7 (FZD7) (AA 161-239) antibody
FZD7 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Frizzled Family Receptor 7 (FZD7) (AA 100-200) antibody (PerCP)
FZD7 适用: 小鼠 WB 宿主: 兔 Polyclonal PerCP

anti-Frizzled Family Receptor 7 (FZD7) (N-Term) antibody
FZD7 适用: 人, 小鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Frizzled Family Receptor 7 (FZD7) (N-Term) antibody
FZD7 适用: 人, 小鼠, 大鼠, 犬, Cow, 兔, Bat, Pig, 猴, Hamster IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Frizzled Family Receptor 7 (FZD7) (C-Term) antibody
FZD7 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Frizzled Family Receptor 7 (FZD7) (C-Term) antibody
Verified FZD7 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated

anti-Frizzled Family Receptor 7 (FZD7) (C-Term) antibody
FZD7 适用: 人, 小鼠, 大鼠, 犬, Cow, 兔, Bat, Pig, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
生效 #029805 (Western Blotting)

by

Alamo Laboratories Inc

No.

#029805

日期

2014.08.26

抗原

Lot Number

120426

Method validated

Western Blotting

Positive Control

MDA-MB-231 cells

Negative Control

C6/36 cells (non-reactive species)

Notes

A strong band was observed in the positive control sample at the expected molecular weight of ~64 kDa. The band is also faintly observed in the negative control sample, which may indicate species cross-reactivity.

Validation Images

Figure 1: Western Blot for FZD7. Arrowhead indicates the expected molecular weight of ~64 kDa.
Full Methods
Primary Antibody

Antigen: Frizzled Family Receptor 7 (FZD7)
Catalog number: ABIN710051
Lot number: 120426
Antibody Dilution: 1:100

Secondary Antibody

Antigen: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate
Lot number: L170-6515
Antibody Dilution: 1:10,000

Full Protocol

1. The cell extracts were heated at 95°C for 5 minutes in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol.
2. 15 μl of heated culture-media were loaded and resolved on 8-16% SDS-polyacrylamide gel.
3. The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
4. Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
5. The PVDF membrane was incubated with 25 ml of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) BSA at room temperature for 1 hour.
6. The membrane was rinsed with TBST once.
7. The membrane was immersed with the protein side up in the primary antibody solution in TBST containing 5% (W/V) BSA and incubated for 24 hours at 4°C.
8. The membrane was rinsed in TBST thrice for 5 minutes each.
9. The membrane was incubated in the HRP-conjugated secondary antibody solution in TBST containing 5% (W/V) BSA and incubated for 1 hour at room temperature (~26°C) with gentle agitation.
10. The membrane was rinsed thrice TBST thrice for 5 minutes each.
11. The membrane was rinsed in TBS twice for 30 seconds each.
12. Signals were detected with ECL-2 Substrate. The blot was scanned for 45 minutes.
13. The membrane was rinsed three times TBST.
14. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 minutes each.
15. The membrane was washed in TBST 2 times for 10 minutes each.
16. Repeated Steps 5-12 with the loading control antibody (for Anti-actin) and its matching secondary antibody.

Experimental Notes

- No experimental challenges noted.
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Deng, Zhang, Zhang, Wen, Miao, Guo: "MicroRNA-142-3p inhibits cell proliferation and invasion of cervical cancer cells by targeting FZD7." in: Tumour biology, (2015) (PubMed).
抗原

FZD7 (Frizzled Family Receptor 7 (FZD7))

别名

Frizzled 7 (FZD7 产品)

别名

FzE3 antibody, Fz-7 antibody, Xfz7 antibody, frizzled-7 antibody, frizzled7 antibody, frz-7 antibody, frz7 antibody, fz7 antibody, fzd7-a antibody, fzd7-b antibody, fze3 antibody, Fz7 antibody, cFz-7 antibody, fb38g02 antibody, fc44b09 antibody, fz13 antibody, fz7a antibody, fz7b antibody, wu:fb38g02 antibody, wu:fc44b09 antibody, zg13 antibody, frizzled class receptor 7 antibody, frizzled class receptor 7 L homeolog antibody, frizzled class receptor 7b antibody, FZD7 antibody, fzd7.L antibody, fzd7 antibody, Fzd7 antibody, fzd7b antibody

背景

Synonyms: FzE3, Frizzled-7, Fz-7, hFz7, FZD7
Background: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.

基因ID

8324

UniProt

O75084

途径

WNT signaling, Stem Cell Maintenance