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GFP 抗体

Name: GFP 抗体
Brand: antibodies-online
SKU: ABIN7141875
Availability: OutOfStock

GFP 适用: Aequorea victoria WB, ELISA, IF, IP, FACS 宿主: 小鼠 Monoclonal 6C11C11 unconjugated

Western Blot Positive WB detected in: 50 ng recombinant protein All lanes: GFP antibody at 1:50000, 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000, 1:3200000, 1:6400000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 32 KDa Observed band size: 32 KDa Exposure time:5 min (GFP 抗体)

Immunofluorescence staining of 293F cells transfected with GFP with ABIN7141875 at 1:100, counter-stained with DAPI. (GFP 抗体)

Western Blot Positive WB detected in: 1,2,3 and 4 are Recombinant proteins with GFP tag for 50 ng, 5, 293F whole cell lysate transfected with GFP for 5 μg All lanes GFP antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size:1,2,3,4 and 5 is 32,32,50,32,32 KDa respectively Observed band size: 32,32,50,32,32 KDa Exposure time:1 min (GFP 抗体)

Overlay histogram showing 293F cells transfected with GFP stained with ABIN7141875 (red line) at 1:200. (GFP 抗体)

FACS

Western Blot Positive WB detected in: Recombinant protein at 25 ng, 12. (GFP 抗体)

Two-color flow cytometric analysis showing 293F cells untransfected (Left) or transfected with GFP (Right) stained with ABIN7141875 at 1:200. (GFP 抗体)

FACS

Immunoprecipitating GFP in 293F whole cell lysate transfected with GFP Lane 1: Mouse control IgG2b instead of ABIN7141875 in 293F whole cell lysate transfected with GFP Lane 2: ABIN7141875 (4 μg) + 293F whole cell lysate transfected with GFP (500 μg) Lane 3: 293F whole cell lysate transfected with GFP (5 μg) For western blotting, the blot was detected with ABIN7141875 at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000 (GFP 抗体)

7 images

产品编号 ABIN7141875

发货至: 中国

Datasheet as PDF

抗原 See all GFP 抗体

GFP (Green Fluorescent Protein (GFP))
适用
174

15

11

9
Aequorea victoria
宿主
63

62

35

14

9

5

1

1
小鼠
Compatible Secondaries
克隆类型
114

71
单克隆
标记
81

12

11

10

5

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is un-conjugated
应用范围
148

74

45

33

25

24

24

21

14

13

13

12

12

5

4

4

2

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

纯化方法

Protein G purified

免疫原

Recombinant GFP Protein

克隆位点

6C11C11

亚型

IgG2b

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应用备注

Recommended dilution: WB:1:50000-1:6400000, IF:1:50-1:200, FC:1:100-1:300, IP:1 μL-2 μL,

限制

仅限研究用
状态

Liquid

缓冲液

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C,-80 °C

储存方法

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
抗原

GFP (Green Fluorescent Protein (GFP))

别名

GFP (GFP 产品)

别名

green fluorescent protein antibody, gfp antibody

背景

Background: The green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. The protein is in the shape of a cylinder, comprising 11 strands of beta-sheet with an alpha-helix inside and short helical segments on the ends of the cylinder. Inward-facing sidechains of the barrel induce specific cyclization reactions in the tripeptide Ser65-Tyr66-Gly67 that lead to chromophore formation. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. The green-fluorescent protein (GFP) of the jellyfish Aequorea victoria has always been used as a universal reporter in a broad range of heterologous living cells and organisms. GFP has become well established as a marker of gene expression and protein targeting in intact cells and organisms.
Aliases: CFP, eGFP, eYFP, GFP, GFP tag, YFP