Glycogen Synthase Kinase 3 (GSK3) (pTyr216), (pTyr279) 抗体
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- 抗原 See all Glycogen Synthase Kinase 3 (GSK3) 抗体
- Glycogen Synthase Kinase 3 (GSK3)
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抗原表位
- pTyr216, pTyr279
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
- 应用范围
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
- 交叉反应
- 人, 小鼠, 大鼠
- 纯化方法
- Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
- 免疫原
- Peptide sequence around phosphorylation site of tyrosine 279/216 (V-S-Y(p)-I-C) derived from Human GSK3alpha/beta .
- 亚型
- IgG
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- 应用备注
- WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200,
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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- 抗原
- Glycogen Synthase Kinase 3 (GSK3)
- 别名
- GSK3A/GSK3B (GSK3 产品)
- 背景
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Background: Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Chin PC, et al. Brain Res Mol Brain Res 2005 Jun 13, 137(1-2): 193-201 Takahashi-Yanaga F, et al. Biochem Biophys Res Commun 2004 Apr 02, 316(2): 411-415 Fan G, et al. J Biol Chem 2003 Dec 26, 278(52): 52432-52436 Liao X, et al. Mol Cancer Ther 2003 Nov, 2(11): 1215-1222
Aliases: Factor A, GSK-3 alpha/beta, kinase GSK3-alpha/beta
- UniProt
- P49840, P49841
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