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MEK2 抗体 (pThr394)

Name: MEK2 抗体 (pThr394)
Brand: antibodies-online
SKU: ABIN7138844
Availability: OutOfStock

MAP2K2 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

Western blot analysis of extracts from HepG2 and Hela cells untreated or treated with UV using MEK-2(Phospho-Thr394) Antibody. (MEK2 抗体 (pThr394))

Immunofluorescence staining of methanol-fixed Hela cells using MEK-2(Phospho-Thr394) Antibody. (MEK2 抗体 (pThr394))

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK-2(Phospho-Thr394) Antibody(left) or the same antibody preincubated with blocking peptide(right). (MEK2 抗体 (pThr394))

IHC

3 images

产品编号 ABIN7138844

发货至: 中国

Datasheet as PDF

抗原 See all MEK2 (MAP2K2) 抗体

MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))
抗原表位
19

14

7

7

7

6

6

6

5

5

3

3

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pThr394
适用
121

68

27

6

5

3

2

2

1
人
宿主
101

19

1

1
兔
Compatible Secondaries
克隆类型
103

19
多克隆
标记
70

7

7

6

5

4

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
This MEK2 antibody is un-conjugated
应用范围
97

48

37

22

20

18

18

15

14

9

6

4

3

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

交叉反应

人, 小鼠, 大鼠

纯化方法

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

免疫原

Peptide sequence around phosphorylation site of threonine 394 (P-G-T(p)-P-T) derived from Human MEK-2.

亚型

IgG

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MAP2K2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 8D10 unconjugated

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MAP2K2 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB14015 unconjugated

应用备注

WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200,

限制

仅限研究用
状态

Liquid

缓冲液

Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C,-80 °C

储存方法

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
抗原

MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))

别名

MAP2K2 (MAP2K2 产品)

别名

CFC4 antibody, MAPKK2 antibody, MEK2 antibody, MKK2 antibody, PRKMK2 antibody, AA589381 antibody, MK2 antibody, Prkmk2 antibody, ATMKK2 antibody, F27B13.50 antibody, F27B13_50 antibody, MAP KINASE KINASE 1 antibody, MAP kinase kinase 2 antibody, MK1 antibody, map2k2 antibody, wu:fa56c06 antibody, zgc:123171 antibody, zgc:172250 antibody, mitogen-activated protein kinase kinase 2 antibody, mitogen activated protein kinase kinase 2 antibody, MAP kinase kinase 2 antibody, mitogen-activated protein kinase kinase 2a antibody, mitogen-activated protein kinase kinase 2b antibody, MAP2K2 antibody, Map2k2 antibody, MKK2 antibody, map2k2a antibody, map2k2b antibody

背景

Background: Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.

Crews C M, et al. (1992) Science. 258:478-480.
Alessi D R, et al. (1994) EMBO J. 13:1610-1619.
Rosen L B, et al. (1994) Neuron. 12:1207-1221.
Cowley S, et al. (1994) Cell. 77:841-852.
Aliases: Cardiofaciocutaneous syndrome antibody, CFC syndrome antibody, CFC4 antibody, Dual specificity mitogen activated protein kinase kinase 2 antibody, Dual specificity mitogen-activated protein kinase kinase 2 antibody, ERK activator kinase 2 antibody, FLJ26075 antibody, MAP kinase kinase 2 antibody, map2k2 antibody, MAPK / ERK kinase 2 antibody, MAPK/ERK kinase 2 antibody, MAPKK 2 antibody, MAPKK2 antibody, MEK 2 antibody, MEK2 antibody, Microtubule associated protein kinase kinase 2 antibody, Mitogen activated protein kinase kinase 2 antibody, Mitogen activated protein kinase kinase 2 p45 antibody, MKK 2 antibody, MKK2 antibody, MP2K2_HUMAN antibody, OTTHUMP00000165826 antibody, OTTHUMP00000165827 antibody, PRKMK 2 antibody, PRKMK2 antibody

UniProt

P36507

途径

MAPK Pathway, RTK signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling