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RASGRF1 抗体 (pSer916)

Name: RASGRF1 抗体 (pSer916)
Brand: antibodies-online
SKU: ABIN7138730
Availability: OutOfStock

RASGRF1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated

Immunohistochemical analysis of paraffin-embedded human brain tissue using Ras-GRF1 (Phospho-Ser916) antibody (left)or the same antibody preincubated with blocking peptide (right). (RASGRF1 抗体 (pSer916))

IHC

1 image

产品编号 ABIN7138730

发货至: 中国

Datasheet as PDF

抗原 See all RASGRF1 抗体

RASGRF1 (Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1))
抗原表位
15

11

7

4

2

2

1

1

1

1

1

1

1

1
pSer916
适用
26

26

23

4

4

4

3

3

3

2

2

1

1
人
宿主
50
兔
Compatible Secondaries
克隆类型
50
多克隆
标记
24

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This RASGRF1 antibody is un-conjugated
应用范围
34

21

14

13

13

7

5

3

1

1

1
Immunohistochemistry (IHC), ELISA

交叉反应

小鼠, 大鼠

纯化方法

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

免疫原

Peptide sequence around phosphorylation site of Serine 916(N-E-G(p)-T P) derived from Human Ras-GRF1.

亚型

IgG

Top Product

Discover our top product RASGRF1 Primary Antibody
anti-Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1) (Middle Region) antibody
RASGRF1 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated

anti-Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1) (AA 1-190) antibody
RASGRF1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1) (N-Term) antibody
RASGRF1 适用: 人 ELISA, WB, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1) (AA 107-156) antibody
RASGRF1 适用: 人, 小鼠, Cow, 犬, 豚鼠, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated

anti-Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1) (AA 467-516) antibody
RASGRF1 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, 斑马鱼, 猴, Pig, Bat, 小鸡 WB 宿主: 兔 Polyclonal unconjugated

anti-Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1) (AA 1-190) antibody
RASGRF1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1) (N-Term) antibody
RASGRF1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1) (AA 907-936) antibody
RASGRF1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB14017 unconjugated

anti-Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1) (N-Term) antibody
RASGRF1 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated

anti-Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1) (pSer929) antibody
RASGRF1 适用: 大鼠 ELISA, WB, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

应用备注

IHC:1:50-1:100,

限制

仅限研究用
状态

Liquid

缓冲液

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C,-80 °C

储存方法

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
抗原

RASGRF1 (Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1))

别名

RASGRF1 (RASGRF1 产品)

别名

CDC25 antibody, CDC25L antibody, GNRP antibody, GRF1 antibody, GRF55 antibody, H-GRF55 antibody, PP13187 antibody, ras-GRF1 antibody, AI844718 antibody, CDC25Mm antibody, Grf1 antibody, Grfbeta antibody, P190-A antibody, Ras-GRF1 antibody, p190 antibody, p190RhoGEF antibody, cdc25 antibody, rasgrf1 antibody, Ras protein specific guanine nucleotide releasing factor 1 antibody, RAS protein-specific guanine nucleotide-releasing factor 1 antibody, Ras protein specific guanine nucleotide releasing factor 1 S homeolog antibody, si:ch211-234p18.3 antibody, RASGRF1 antibody, Rasgrf1 antibody, rasgrf1.S antibody, si:ch211-234p18.3 antibody, rasgrf1 antibody

背景

Background:
Ras activity is regulated by GAP (GTPase activating proteins) and GEFs (guanine nucleotide exchange factors). Ras-GRF1 (also known as CDC25Mm) is neuronal RasGEF and is regulated by heterotrimeric G proteins and calcium influx . Binding to calmodulin and phosphorylation stimulate Ras-GRF1 activity. Multiple PKA phosphorylation sites on Ras-GRF have been identified. Phosphorylation on the two major sites, Ser54 and Ser822, inhibits Ras-GRF activity. Carbachol (a muscarinic agonist)-induced phosphorylation on Ser916 is essential but not sufficient for maximal Ras-GRF activity.

Huibin Yang, J. Biol. Chem., Apr 2003, 278: 13278 - 13285.
Huibin Yang, Mol. Biol. Cell, May 2006, 17: 2177 - 2189.
Raymond R. Mattingly, J. Biol. Chem., Dec 1999, 274: 37379.
Tie-Nian Zhu, J. Biol. Chem., May 2007, 282: 14816 - 14826.
Aliases: CDC25 antibody, CDC25L antibody, GNRP antibody, GRF1 antibody, GRF55 antibody, Guanine nucleotide exchange factor antibody, Guanine nucleotide releasing factor 55 kD antibody, Guanine nucleotide releasing protein antibody, guanine nucleotide-releasing factor 1 antibody, Guanine nucleotide-releasing protein antibody, H GRF55 antibody, HGRF55 antibody, PP13187 antibody, Ras protein specific guanine nucleotide releasing factor 1 antibody, Ras specific guanine nucleotide releasing factor antibody, Ras specific guanine nucleotide releasing factor CDC25 homolog antibody, Ras specific nucleotide exchange factor CDC25 antibody, Ras-GRF1 antibody, Ras-specific guanine nucleotide-releasing factor 1 antibody, Ras-specific nucleotide exchange factor CDC25 antibody, RASGRF1 antibody, RGRF1_HUMAN antibody

UniProt

Q13972

途径

RTK signaling, Cell Division Cycle, M Phase