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CHEK1 抗体 (pSer296)

Name: CHEK1 抗体 (pSer296)
Brand: antibodies-online
SKU: ABIN7138533
Availability: OutOfStock

CHEK1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

Western blot analysis of extracts from HUVEC cells treated with UV using Chk1 (Phospho-Ser296) Antibody. (CHEK1 抗体 (pSer296))

Immunohistochemical analysis of paraffin-embedded human brain tissue using Chk1 (Phospho-Ser296) antibody (left)or the same antibody preincubated with blocking peptide (right). (CHEK1 抗体 (pSer296))

IHC

2 images

产品编号 ABIN7138533

发货至: 中国

Datasheet as PDF

抗原 See all CHEK1 抗体

CHEK1 (Checkpoint Kinase 1 (CHEK1))
抗原表位
36

27

19

15

9

7

6

6

5

4

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer296
适用
182

118

68

16

11

9

6

6

6

4

4

3

2

1

1
人
宿主
192

14

6

5
兔
Compatible Secondaries
克隆类型
194

24
多克隆
标记
124

11

9

8

4

4

4

4

4

4

4

4

4

3

3

3

3

3

3

3

3

3

3
This CHEK1 antibody is un-conjugated
应用范围
166

88

53

41

40

36

32

15

11

10

7

6

3

3

2

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

交叉反应

人

纯化方法

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

免疫原

Peptide sequence around phosphorylation site of Serine 296(I-F-S(p)-N-L) derived from Human Chk1.

亚型

IgG

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anti-Checkpoint Kinase 1 (CHEK1) (pSer296) antibody
CHEK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

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CHEK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (pSer301) antibody
CHEK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (pSer345) antibody
CHEK1 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (Middle Region) antibody
CHEK1 适用: 人, 小鼠, 大鼠, 犬, 兔, 马, Cow, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody
CHEK1 适用: 小鼠, 小鸡 ELISA, IHC (p), ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) antibody
CHEK1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Monoclonal 2F2 unconjugated Recombinant Antibody

anti-Checkpoint Kinase 1 (CHEK1) antibody
CHEK1 适用: 人, 小鼠 WB, ELISA 宿主: 小鼠 Monoclonal 2G1D5 unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody
CHEK1 适用: 人 WB, IHC (p), DB 宿主: 兔 Polyclonal RB7845-RB18425 unconjugated

应用备注

WB:1:500-1:1000, IHC:1:50-1:100,

限制

仅限研究用
状态

Liquid

缓冲液

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C,-80 °C

储存方法

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
抗原

CHEK1 (Checkpoint Kinase 1 (CHEK1))

别名

CHEK1 (CHEK1 产品)

别名

An08g10320 antibody, AO090003000441 antibody, CHK1 antibody, chk1 antibody, C85740 antibody, Chk1 antibody, rad27 antibody, id:ibd2720 antibody, zgc:56093 antibody, CHEK1 antibody, checkpoint kinase 1 antibody, serine/threonine-protein kinase chk1 antibody, serine/threonine-protein kinase Chk1 antibody, CAMK/CAMKL/CHK1 protein kinase Chk1 antibody, checkpoint kinase 1 S homeolog antibody, CHEK1 antibody, ANI_1_2436074 antibody, AOR_1_768154 antibody, PTRG_04183 antibody, SJAG_01680 antibody, PAAG_04978 antibody, MCYG_03290 antibody, VDBG_03742 antibody, chek1.S antibody, Chek1 antibody, chek1 antibody

背景

Background:
Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation.

Sanchez Y., Science 277:1497-1501(1997).
Flaggs G., Curr. Biol. 7:977-986(1997).
Semba S., Int. J. Oncol. 16:731-737(2000).

Aliases: C85740 antibody, Cell cycle checkpoint kinase antibody, Checkpoint , S. pombe, homolog of, 1 antibody, Checkpoint kinase 1 antibody, Checkpoint kinase 1 homolog (S. pombe) antibody, CHEK 1 antibody, Chek1 antibody, Chk 1 antibody, Chk1 antibody, CHK1 checkpoint homolog (S. pombe) antibody, CHK1_HUMAN antibody, EC 2.7.11.1 antibody, rad27 antibody, Serine/threonine protein kinase Chk1 antibody, Serine/threonine-protein kinase CHK1 antibody, STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) antibody

UniProt

O14757

途径

p53 Pathway, Apoptosis, Cell Division Cycle, DNA Damage Repair