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His Tag 抗体

Name: His Tag 抗体
Brand: antibodies-online
SKU: ABIN7131650
Availability: OutOfStock

WB, ELISA 宿主: 小鼠 Monoclonal 3G5H8 unconjugated

Western Blot Positive WB detected in: HIS fusion protein 1 at 20 ng, 10 ng, 5 ng, 2. (His Tag 抗体)

Western Blot Positive WB detected in: HIS fusion protein 2 at 20 ng, 10 ng, 5 ng, 2. (His Tag 抗体)

2 images

产品编号 ABIN7131650

发货至: 中国

Datasheet as PDF

抗原 See all His Tag 抗体

His Tag

适用

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宿主
92

60

11

9

5

4

1

1
小鼠
Compatible Secondaries
克隆类型
94

76

10
单克隆
标记
78

21

14

13

5

4

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This His Tag antibody is un-conjugated
应用范围
141

91

39

27

25

19

15

14

14

13

13

7

7

5

5

4

4

4

3

3

2

2

1

1

1
Western Blotting (WB), ELISA

纯化方法

Protein A purified

免疫原

HHHHHH(6x His) synthetic peptide conjugate to KLH

克隆位点

3G5H8

亚型

IgG2a

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WB, ELISA, IP 宿主: 小鼠 Monoclonal 33D10-D2 HRP

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WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-His Tag antibody (Biotin)
WB, ELISA, IP 宿主: 小鼠 Monoclonal 33D10-D2 Biotin

anti-His Tag antibody
WB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal HIS-H8 unconjugated

anti-His Tag antibody (DyLight 649)
WB, FM, FLISA 宿主: 小鼠 Monoclonal 33D10-D2 DyLight 649

应用备注

Recommended dilution: WB:1:500-1:2000,

限制

仅限研究用
状态

Liquid

缓冲液

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C,-80 °C

储存方法

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
抗原

His Tag

别名

6*His (His Tag 产品)

物质类

Tag

背景

Background: A His tag is a tag of 6 histidines added to the end of the protein that the DNA codes for, so the DNA with contain 6 repeats of the His codon. What it is used for is purifying the protein that the DNA codes for. Histidines are positively charged amino acids and a group of 6 of them allows the protein to bind to a negatively charged column. Histidines are also special in that they have a pKa around 7, meaning that once bound to the column they can easily be unbound.
Aliases: 6*His, 6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His Tag