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beta Actin 抗体 (AA 1-50)

Name: beta Actin 抗体 (AA 1-50)
Brand: antibodies-online
SKU: ABIN7127368
Availability: OutOfStock

ACTB 适用: 人 WB, ELISA, IHC, IF, IP, FACS 宿主: 小鼠 Monoclonal 1D12E12B5 unconjugated

Western Blot Positive WB detected in: Rabbit liver tissue, Rabbit brain tissue, Rabbit lung tissue, Rabbit kidney tissue, Rabbit stomach tissue, A549 whole cell lysate All lanes: ACTB antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time: 5 min (beta Actin 抗体 (AA 1-50))

IHC image of ABIN7127368 diluted at 1:500 and staining in paraffin-embedded breast cancer performed on a Leica BondTM system. (beta Actin 抗体 (AA 1-50))

IHC

Western Blot Positive WB detected in: Mouse liver tissue, Mouse brain tissue, Mouse lung tissue, Mouse kidney tissue, Mouse spleen tissue, Mouse stomach tissue All lanes: ACTB antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time: 5 min (beta Actin 抗体 (AA 1-50))

Western Blot Positive WB detected in: Rat liver tissue, Rat brain tissue, Rat lung tissue, Rat stomach tissue, U87 whole cell lysate All lanes: ACTB antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time: 5 min (beta Actin 抗体 (AA 1-50))

Western Blot Positive WB detected in: 293T whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, Mouse kidney tissue, Rabbit kidney tissue All lanes: ACTB antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time: 5 min (beta Actin 抗体 (AA 1-50))

Western Blot Positive WB detected in: 293T whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, Rat spleen tissue, Rabbit kidney tissue All lanes: ACTB antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time: 5 min (beta Actin 抗体 (AA 1-50))

Western Blot Positive WB detected in: 293T whole cell lysate at 40 μg, 20 μg, 10 μg All lanes:ACTB antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time: 5 min (beta Actin 抗体 (AA 1-50))

Western Blot Positive WB detected in: 20 μg 293T whole cell lysate ACTB antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time: 5 min (beta Actin 抗体 (AA 1-50))

IHC image of ABIN7127368 diluted at 1:500 and staining in paraffin-embedded colon cancer performed on a Leica BondTM system. (beta Actin 抗体 (AA 1-50))

IHC

9 images

产品编号 ABIN7127368

发货至: 中国

Datasheet as PDF

抗原 See all beta Actin (ACTB) 抗体

beta Actin (ACTB) (Actin, beta (ACTB))
抗原表位
39

15

14

6

6

5

5

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 1-50
适用
174

125

107

55

43

41

37

25

23

22

18

17

12

10

8

7

7

6

6

6

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1
人
宿主
113

76

5

1

1
小鼠
Compatible Secondaries
克隆类型
111

83
单克隆
标记
122

12

12

10

3

3

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This beta Actin antibody is un-conjugated
应用范围
163

68

50

45

35

31

25

21

13

13

8

6

6

5

5

2

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

交叉反应

人, 小鼠, 兔, 大鼠

纯化方法

Protein A purified

免疫原

A synthesized peptide derived from human Beta-Actin (1-50AA)

克隆位点

1D12E12B5

亚型

IgG2b

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anti-Actin, beta (ACTB) antibody
ACTB 适用: 人 WB, IF, IP 宿主: 小鼠 Monoclonal unconjugated

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ACTB 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Actin, beta (ACTB) (AA 2-16) antibody
ACTB 适用: 小鼠, 大鼠, 小鸡, 斑马鱼 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated

anti-Actin, beta (ACTB) (AA 1-25) antibody
ACTB 适用: 人, 小鼠, 大鼠, Pig, Cow WB, IHC (p) 宿主: 小鼠 Monoclonal 1A2 unconjugated

anti-Actin, beta (ACTB) (AA 1-375) antibody
ACTB 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3G4-F9 unconjugated

anti-Actin, beta (ACTB) (AA 1-375) antibody
ACTB 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Actin, beta (ACTB) (N-Term) antibody
KO Validated ACTB 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

anti-Actin, beta (ACTB) antibody
ACTB 适用: 人, 小鼠, 小鸡, 兔, Pig, Cow, Hamster, 山羊, 鱼 WB, ELISA 宿主: 小鼠 Monoclonal 2D1D10 unconjugated

anti-Actin, beta (ACTB) antibody
ACTB 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p), FACS, DNA Mic 宿主: 小鼠 Monoclonal 137CT26-1-1 unconjugated

应用备注

Recommended dilution: WB: 1:5000-1:80000, IHC: 1:500-1:1000, IF: 1:50-1:200, FC: 1:100-1:300, IP: 1 µL-5 µL,

限制

仅限研究用
状态

Liquid

缓冲液

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C,-80 °C

储存方法

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
抗原

beta Actin (ACTB) (Actin, beta (ACTB))

别名

ACTB (ACTB 产品)

背景

ACTB,ACTB,ACTB,PS1TP5BP1,beta-ac6n,β-ac6n

UniProt

P60709

途径

Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction