Recombinant Keratin 77 抗体 (AA 100-200)
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- 抗原 See all Keratin 77 (KRT77) 抗体
- Keratin 77 (KRT77)
- 抗体类型
- Recombinant Antibody
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抗原表位
- AA 100-200
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Keratin 77 antibody is un-conjugated
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应用范围
- Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- The keratins are the typical intermediate filament proteins of epithelia, showing an outstanding degree of molecular diversity. Heteropolymeric filaments are formed by pairing of type I and type II molecules. In humans 54 functional keratin genes exist. They are expressed in highly specific patterns related to the epithelial type and stage of cellular differentiation. This antibody can detect high molecular weight CK10, CK14, CK15 and CK16 and low molecular weight CK19. It usually used in a cytokeratin cocktail with type II cytokeratin antibody.
- 交叉反应 (详细)
- Human.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Synthetic peptide corresponding to Type I Cytokeratins residues within aa100-200 of Type I Cytokeratins
- 亚型
- IgG1
- Top Product
- Discover our top product KRT77 Primary Antibody
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- 应用备注
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Skin or squamous cell carcinoma.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- Keratin 77 (KRT77)
- 别名
- KRT77 (KRT77 产品)
- 别名
- K1B antibody, KRT1B antibody, 4732484G22Rik antibody, K1emb antibody, Krt239 antibody, mK1b antibody, Kb39 antibody, keratin 77 antibody, KRT77 antibody, Krt77 antibody
- 背景
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K1B, KRT1B, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B,Cytokeratin, Type I (Epithelial Marker)
Cellular localisation: Cytoplasmic - 分子量
- 60/53/51/45/40kDa
- 基因ID
- 374454, 334989
- UniProt
- Q7Z794
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