Recombinant Histone H3.3 抗体
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- 抗原 See all Histone H3.3 (H3F3A) 抗体
- Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))
- 抗体类型
- Recombinant Antibody
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Histone H3.3 antibody is un-conjugated
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应用范围
- Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- Phosphohistone H3 (PHH3) is a marker specific for cells undergoing mitosis. Serine 10 of Histone H3 is phosphorylated in association with mitotic chromatin condensation in late G2 and M phase of the cell cycle and thus, PHH3 can distinguish mitosis from apoptotic nuclei. The range of percentage PHH3 positive tumor nuclei was from 0.0 to 6.6% (median value 0.8%). Increased expression of PHH3was significantly associated with tumor thickness (p = 0.031), presence of tumor ulceration (p =0.041) and tumor necrosis (p =0.027), but not with Clark's level of invasion. High levels of PHH3 was associated with increased mitotic count (p = 0.003) and high Ki-67 expression (p = 0.002). For central nervous system tumors, melanoma, soft tissue tumors, GIST, etc., PHH3 mAb is helpful for tumor pathological classification and prognosis.
- 交叉反应 （详细）
- Human.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- A synthetic peptide corresponding to (ARK-pS-TGGKAPRKQLc) of Phosphohistone H3 (phospho S10)
- 亚型
- IgG1
- Top Product
- Discover our top product H3F3A Primary Antibody
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anti-H3 Histone, Family 3A (H3F3A) (AA 2-136) antibody
H3F3A 适用: 人 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-H3 Histone, Family 3A (H3F3A) (Lys9) antibodyH3F3A 适用: 人 ELISA, WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-H3 Histone, Family 3A (H3F3A) (pThr3) antibodyH3F3A 适用: 人 ELISA, WB, FACS, ICC 宿主: 兔 Monoclonal 28H4 unconjugated Recombinant Antibody
anti-H3 Histone, Family 3A (H3F3A) antibodyH3F3A 适用: 人 ELISA, IHC, IF 宿主: 兔 Monoclonal 4H2 unconjugated Recombinant Antibody
anti-H3 Histone, Family 3A (H3F3A) antibodyH3F3A 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-H3 Histone, Family 3A (H3F3A) (2meLys79) antibodyH3F3A 适用: 人 ELISA, WB, ChIP 宿主: 兔 Polyclonal unconjugated
anti-H3 Histone, Family 3A (H3F3A) antibodyMicroarray verified H3F3A 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB199456 unconjugated
anti-H3 Histone, Family 3A (H3F3A) antibodyMicroarray verified H3F3A 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB267809 unconjugated
anti-H3 Histone, Family 3A (H3F3A) (AA 1-30) antibodyH3F3A 适用: 人 ELISA, DB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Human tonsil, liver or breast carcinoma.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))
- 别名
- H3F3 (H3F3A 产品)
- 别名
- H3.3A antibody, H3F3 antibody, h3f3a antibody, MGC52829 antibody, wu:fb58e10 antibody, zgc:56193 antibody, zgc:86731 antibody, H3-I antibody, H3-II antibody, H3-III antibody, H3-IV antibody, H3-V antibody, H3-VI antibody, H3-VII antibody, H3-VIII antibody, H3F3A antibody, h3.3 antibody, h3.3a antibody, CG5825 antibody, Dmel\\CG5825 antibody, H3.3 antibody, His-3.3A antibody, His3.3 antibody, PH3 antibody, dH3.3A antibody, H3 histone family member 3A antibody, H3 histone, family 3A antibody, H3 histone, family 3A L homeolog antibody, H3 histone, family 3C antibody, histone H3.3 antibody, histone cluster 1, H3a antibody, Histone H3.3A antibody, H3F3A antibody, h3f3a.L antibody, h3f3a antibody, H3F3C antibody, LOC475916 antibody, LOC480110 antibody, HIST1H3A antibody, LOAG_05159 antibody, His3.3A antibody, H3f3a antibody
- 背景
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Hist1h3a, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, Histone cluster 1, H3a, Histone H3 3 pseudogene, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l,Histone H3, phosphorylated (pSer10) (Nuclear Marker)
Cellular localisation: Nucleus. Chromosome. - 分子量
- 15kDa
- 基因ID
- 8350, 132854
- UniProt
- P68431
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