UBE2B 抗体
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- 抗原 See all UBE2B 抗体
- UBE2B (Ubiquitin-Conjugating Enzyme E2B (UBE2B))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This UBE2B antibody is un-conjugated
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应用范围
- Immunofluorescence (IF), Flow Cytometry (FACS)
- 特异性
- The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires the ATP-dependent activation of the Ub C-terminus and the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. UBE2A (ubiquitin-conjugating enzyme E2 A) and UBE2B (ubiquitin-conjugating enzyme E2 B) are both Ub-conjugating enzymes that are essential to post replication repair of UV-damaged DNA. UBE2A and UBE2B are both nuclear and cell membrane proteins that have been found to interact with Rad18.
- 交叉反应 (详细)
- Human. Predicted to work in Mouse, Rat, Xenopus and Zebrafish.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Recombinant full-length human UBE2B protein
- 亚型
- IgG2b
- Top Product
- Discover our top product UBE2B Primary Antibody
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- 应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.
Positive_Control: HeLa, K562 or Jurkat cells.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- UBE2B (Ubiquitin-Conjugating Enzyme E2B (UBE2B))
- 别名
- UBE2B (UBE2B 产品)
- 别名
- E2-17kDa antibody, HHR6B antibody, HR6B antibody, RAD6B antibody, UBC2 antibody, e2-17kda antibody, hhr6b antibody, hr6b antibody, rad6b antibody, ubc2 antibody, zgc:100921 antibody, 2610301N02Rik antibody, E2-14k antibody, Rad6b antibody, mHR6B antibody, ubiquitin conjugating enzyme E2 B antibody, ubiquitin-conjugating enzyme E2B antibody, ubiquitin-conjugating enzyme E2B (RAD6 homolog) antibody, ubiquitin conjugating enzyme E2 B S homeolog antibody, UBE2B antibody, Ube2b antibody, ube2b antibody, ube2b.S antibody
- 物质类
- Viral Protein
- 背景
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E2 17 kDa, hHR6B, HR6B, RAD6 homolog B (RAD6B), UBC2, UBE2B, Ubiquitin carrier protein B, Ubiquitin conjugating enzyme E2B, Ubiquitin protein ligase B, Ubiquitin-conjugating enzyme E2 17 kDa,UBE2B
Cellular localisation: Cell membrane. Nucleus. In peripheral neurons, expressed both at the plasma membrane and in nuclei. - 分子量
- 17kDa
- 基因ID
- 7320
- UniProt
- P63146
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