SP100 抗体
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- 抗原 See all SP100 抗体
- SP100 (SP100 Nuclear Antigen (SP100))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This SP100 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Coating (Coat), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- The human SP100 gene encodes an autoantigen that co-localizes with PML and NDP52 in distinct nuclear domains, called nuclear dots (NDs) or ND10 nuclear bodies. Papova-, adeno-, and herpesviruses begin their transcription and DNA-replication at NDs, which play a role in autoimmunity, viral infections and in the etiology of acute promyelocytic leukemia. SP-100 is an interferon inducible protein that has two splice variants. One splice variant contains a highly conserved copy of the DNA-binding high mobility group 1 protein sequence, and thus represents a novel HMG-box protein. This alternatively spliced variant of SP-100 has a unique expression and localization pattern that is distinct from the SP-100 full-length protein. The SP100 protein is covalently modified by the small ubiquitin-related protein SUMO-1. SP-100 contains a functional nuclear localization signal and an ND-targeting region, which overlaps with the SP-100 homodimerization domain.
- 交叉反应 (详细)
- Human.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Recombinant full-length human SP100 protein
- 亚型
- IgG2b
- Top Product
- Discover our top product SP100 Primary Antibody
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- 应用备注
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Known_Application: ELISA (For coating, order antibody without BSA), Western Blot (1-2 μg/mL), Immunofluorescence (0.5-2 μg/mL),Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), Flow Cytometry (0.5-2 μg/million cells), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: HeLa or U87 cells.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- SP100 (SP100 Nuclear Antigen (SP100))
- 别名
- SP100 (SP100 产品)
- 别名
- SP100 antibody, A430075G10Rik antibody, lysp100b antibody, SP140 antibody, SP100 nuclear antigen antibody, nuclear antigen Sp100 antibody, nuclear autoantigen Sp-100 antibody, SP100 antibody, Sp100 antibody, LOC101120993 antibody
- 背景
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Lysp100b, Nuclear antigen Sp100, Nuclear autoantigen Sp 100, Nuclear dot associated Sp100 protein, SP100, SP100 HMG nuclear autoantigen, SP100 nuclear antigen, Speckled 100 kDa,SP100
Cellular localisation: Nucleus. PML body. - 分子量
- 100.4kDa
- 基因ID
- 6672, 369056
- UniProt
- P23497
- 途径
- Retinoic Acid Receptor Signaling Pathway
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