Keratin 3 抗体
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- 抗原 See all Keratin 3 (KRT3) 抗体
- Keratin 3 (KRT3)
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适用
- 兔
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Keratin 3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins, which are arranged in pairs of heterotypic keratin chains co-expressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the corneal epithelium with family member KRT12 and mutations in these genes have been associated with Meesmann's Corneal Dystrophy.KRT3/2542 recognizes the 64kD polypeptide/keratin K3 (cytokeratin 3) of rabbit corneal epithelium and keratin K76 (cytokeratin K2p) of palate epithelium. As such represents an excellent marker for studying corneal epithelial-type differentiation. Also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line.
- 交叉反应 (详细)
- Human, rabbit and bovine.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Tissue, cells or virus corresponding to Rabbit Cytokeratin 3/CK-3. Rabbit corneal epithelial keratin.
- 亚型
- IgG1
- Top Product
- Discover our top product KRT3 Primary Antibody
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- 应用备注
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Known_Application: Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: A431 cells. Human cornea.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- Keratin 3 (KRT3)
- 别名
- KRT3 (KRT3 产品)
- 别名
- CK3 antibody, K3 antibody, CK-3 antibody, keratin 3 antibody, KRT3 antibody
- 背景
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65 KDa Cytokeratin, CK3, CK-3, cytokeratin-3, Keratin, keratin 3, Keratin, Type II Cytoskeletal 3, Keratin-3, Type II Cytoskeletal 3, Type-II keratin Kb3,Cytokeratin 3 (KRT3) (Corneal Epithelial Marker)
Cellular localisation: Cytoplasmic - 分子量
- 65kDa
- 基因ID
- 38550, 680652
- UniProt
- P12035
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