UBE2C 抗体
Quick Overview for UBE2C 抗体 (ABIN7504126)
抗原
See all UBE2C 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires the ATP-dependent activation of the Ub C-terminus and the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ubconjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. UBE2C, also designated UBCH10 in human, is an E2 ubiquitin conjugating enzyme for the anaphase promoting complex (APC), which coordinates mitosis and G1 by sequentially promoting the degradation of key cell-cycle regulators. UBE2C is overexpressed in many different types of cancers and may be a potential therapeutic target.
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交叉反应 （详细）
- Human
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纯化方法
- 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
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免疫原
- Recombinant human full-length UBE2C protein
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亚型
- IgG2b
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anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 70-179) antibody
UBE2C 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 9D3 unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 1-179) antibodyUBE2C 适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 1F5D3 unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 1-179) antibodyUBE2C 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (C-Term) antibodyVerified UBE2C 适用: 人 WB, ELISA, IF, IP, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 2-179) antibodyUBE2C 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 1-179) antibodyUBE2C 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3B1 unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) antibodyUBE2C 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 2-179) antibodyUBE2C 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 1-179) antibodyUBE2C 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2C (UBE2C) (AA 25-129) antibodyUBE2C 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D8 unconjugated
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应用备注
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Positive Control: HeLa, Jurkat or A431 cells. Human tonsil or placenta tissue.
Known Application: ELISA (For coating, order antibody without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.
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有效期
- 24 months
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- UBE2C (Ubiquitin-Conjugating Enzyme E2C (UBE2C))
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别名
- UBE2C
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背景
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Cyclin selective ubiquitin carrier protein, mitotic specific ubiquitin conjugating enzyme, UB E2C, UBCH10, Ubiquitin protein ligase C,Ubiquitin-conjugating Enzyme E2C / UBCH10
Cellular localisation: Cytoplasm. -
分子量
- 20kDa
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基因ID
- 11065, 93002
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UniProt
- O00762
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途径
- Ubiquitin Proteasome Pathway
抗原
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