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TDRKH 抗体

TDRKH 适用: 人 WB, IF, IP, FACS, IHC (f) 宿主: 小鼠 Monoclonal unconjugated
产品编号 ABIN7504177
发货至: 中国
  • 抗原 See all TDRKH products
    TDRKH (Tudor and KH Domain Containing (TDRKH))
    适用
    • 11
    • 5
    • 5
    宿主
    • 8
    • 2
    • 1
    小鼠
    克隆类型
    • 10
    • 1
    单克隆
    标记
    • 9
    • 1
    • 1
    This TDRKH antibody is un-conjugated
    应用范围
    • 10
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    特异性
    Participates in the primary piRNA biogenesis pathway and is required during spermatogenesis to repress transposable elements and prevent their mobilization, which is essential for the germline integrity. The piRNA metabolic process mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Required for the final steps of primary piRNA biogenesis by participating in the processing of 31-37 nt intermediates into mature piRNAs. May act in pi-bodies and piP-bodies by transferring piRNA precursors or intermediates to or between these granules.
    交叉反应 (详细)
    Human. Predicted to react with Mouse and Rat.
    纯化方法
    200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
    免疫原
    Recombinant full-length human TDRKH protein
    亚型
    IgG2b
  • 应用备注

    Positive Control: HeLa or MCF7 cells. Human testis or brain.

    Known Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    限制
    仅限研究用
  • 浓度
    200 μg/mL
    缓冲液
    Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-80 °C
    储存方法
    Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.
    有效期
    24 months
  • 抗原
    TDRKH (Tudor and KH Domain Containing (TDRKH))
    别名
    TDRKH (TDRKH 产品)
    别名
    RGD1311196 antibody, tdrd2 antibody, MGC85144 antibody, TDRD2 antibody, 2700091C21Rik antibody, Tdrd2 antibody, tudor and KH domain containing antibody, tudor and KH domain containing L homeolog antibody, tudor and KH domain containing protein antibody, Tdrkh antibody, TDRKH antibody, tdrkh.L antibody, tdrkh antibody
    背景
    2700091C21Rik, Putative RNA binding protein, RGD1311196, RP11-98D18.8, TDRD2, TDRD7, Tdrkh, Tudor and KH domain containing, Tudor and KH domain-containing protein, tudor domain containing 2,TDRKH
    Cellular localisation: Cytoplasm. Localized to cytosol and centrosome.
    分子量
    62.05kDa
    基因ID
    11022, 144439
    UniProt
    Q9Y2W6
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