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Ube2t 抗体

This anti-Ube2t antibody is a 兔 多克隆 antibody detecting Ube2t in WB, ELISA 和 IP. Suitable for 人, 小鼠 和 大鼠.
产品编号 ABIN7120563
发货至: 中国

Quick Overview for Ube2t 抗体 (ABIN7120563)

抗原

See all Ube2t 抗体
Ube2t (Ubiquitin-Conjugating Enzyme E2T (Ube2t))

适用

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人, 小鼠, 大鼠

宿主

  • 34
  • 4
  • 1

克隆类型

  • 34
  • 5
多克隆

标记

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This Ube2t antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunoprecipitation (IP)
  • 纯化方法

    Immunogen affinity purified

    纯度

    ≥95 % as determined by SDS-PAGE

    免疫原

    ubiquitin-conjugating enzyme E2T(putative)

    亚型

    IgG
  • 应用备注

    WB: 1:200-1:2000, IP: 1:200-1:2000

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

    有效期

    12 months
  • 抗原

    Ube2t (Ubiquitin-Conjugating Enzyme E2T (Ube2t))

    别名

    UBE2T/HSPC150

    背景

    Synonyms:HSPC150, PIG50, UBE2T, Ubiquitin carrier protein T, Ubiquitin protein ligase T Background:The ubiquitin(Ub)-mediated protein degradation pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub to specific protein substrates. The first step requires ATP-dependent activation of the C-terminus of Ub and the assembly of multi-Ubs by Ub-activating enzyme E1. The ubiquitin-conjugating enzyme E2, catalytic(UBCc) domain, is then conjugated to Ubs, through a thiol-ester linkage between a conserved cysteine and the C-terminus of Ub, to generate an intermediate Ub-E2 complex. Then the E3, a ligase, catalyzes the transfer of Ub from E2 to the appropriate substrate. This pathway regulates many fundamental cellular processes. There are also other E2s which form thiol-ester linkages without the use of E3s as well as several UBC homologs(TSG101, Mms2, Croc-1 and similar proteins), which lack the active site cysteine essential for ubiquitination and appear to function in DNA repair pathways.

    分子量

    24 kDa

    基因ID

    28089

    UniProt

    Q9NPD8

    途径

    Chromatin Binding
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