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Layilin 抗体 (AA 281-382)

Name: Layilin 抗体 (AA 281-382)
SKU: ABIN707756
Availability: OutOfStock

LAYN 适用: 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Paraformaldehyde-fixed, paraffin embedded mouse placenta, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30min, Antibody incubation with (Layilin) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37°C, followed by a conjugated streptavidin for 40 minutes and DAPI staining of the nuclei. (Layilin 抗体 (AA 281-382))

IF (p)

Paraformaldehyde-fixed, paraffin embedded Mouse small intestine tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30min, Antibody incubation with Layilin Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining (Layilin 抗体 (AA 281-382))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded mouse intestines, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30min, Antibody incubation with (Layilin) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37°C, followed by a conjugated streptavidin for 40 minutes and DAPI staining of the nuclei. (Layilin 抗体 (AA 281-382))

IF (p)

3 images

产品编号 ABIN707756

发货至: 中国

Datasheet as PDF

抗原 See all Layilin (LAYN) 抗体

Layilin (LAYN)
抗原表位
4

3

2

2

2

2

2

1
AA 281-382
适用
25

11

5

2

1

1

1

1

1
小鼠
宿主
18

7
兔
Compatible Secondaries
克隆类型
20

5
多克隆
标记
15

2

1

1

1

1

1

1

1

1
This Layilin antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

小鼠

预测反应

Human,Rat,Dog,Cow,Horse,Chicken,Rabbit

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human Layilin

亚型

IgG

Top Product

Discover our top product LAYN Primary Antibody
anti-Layilin (LAYN) (Internal Region) antibody
LAYN 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Layilin (LAYN) (AA 1-374) antibody
LAYN 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated

anti-Layilin (LAYN) (Internal Region) antibody
LAYN 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Layilin (LAYN) (AA 262-382) antibody
LAYN 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Layilin (LAYN) (AA 41-90) antibody
LAYN 适用: 人, 小鼠, 大鼠, 豚鼠, Pig WB 宿主: 兔 Polyclonal unconjugated

anti-Layilin (LAYN) (N-Term) antibody
LAYN 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Layilin (LAYN) (Internal Region) antibody
LAYN 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Layilin (LAYN) (AA 262-382) antibody (HRP)
LAYN 适用: 人 ELISA 宿主: 兔 Polyclonal HRP

anti-Layilin (LAYN) (AA 262-382) antibody (FITC)
LAYN 适用: 人宿主: 兔 Polyclonal FITC

anti-Layilin (LAYN) (AA 262-382) antibody (Biotin)
LAYN 适用: 人 ELISA 宿主: 兔 Polyclonal Biotin

应用备注

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
抗原

Layilin (LAYN)

别名

Layilin (LAYN 产品)

别名

LAYN antibody, si:dkey-27p23.2 antibody, E030012M19Rik antibody, Gm511 antibody, RGD1564444 antibody, layilin antibody, layilin a antibody, LAYN antibody, layna antibody, Layn antibody

背景

Synonyms: Layn, E030012M19Rik, Gm511.
Background: LAYN is integral membrane protein, and a hyaluronate receptor. It has been shown to bind specifically to hyaluronan but not to other glycosaminoglycans. It is a recently characterized transmembrane protein with homology to C type lectins, and is a membrane binding site for talin in peripheral ruffles of spreading cells. LAYN may have a role in cell adhesion and motility.

基因ID

143903