我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

AQP2 抗体 (AA 171-271)

Name: AQP2 抗体 (AA 171-271)
SKU: ABIN707576
Availability: OutOfStock
Rating: 5 (1 reviews)

AQP2 适用: 人, 大鼠, 小鼠 WB, ELISA, IF (p), IF (cc), IHC (p), ICC, FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry validation image for anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 171-271) antibody (ABIN707576) (AQP2 抗体 (AA 171-271))

IHC

Mouse kidney lysates probed with Anti-AQP2 Polyclonal Antibody, Unconjugated at 1:5000 90min in 37˚C. (AQP2 抗体 (AA 171-271))

Independently Validated Antibody, image provided by Science Direct, badge number 029457:Formalin-fixed and paraffin embedded human kidney labeled with Anti-AQP2 Polyclonal Antibody, Unconjugated (ABIN707576) at 1:250 followed by conjugation to the secondary antibody and DAB staining (AQP2 抗体 (AA 171-271))

12 images

(2 references)

(1 validation)

产品编号 ABIN707576

发货至: 中国

Datasheet as PDF

抗原 See all AQP2 抗体

AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))
抗原表位
21

20

17

16

15

15

7

5

5

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1
AA 171-271
适用
108

95

79

15

15

10

5

4

3

2

2

2

1

1
人, 大鼠, 小鼠
宿主
130

2
兔
Compatible Secondaries
克隆类型
131

2
多克隆
标记
46

10

9

6

4

4

4

4

4

4

4

4

4

4

4

3

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This AQP2 antibody is un-conjugated
应用范围
115

40

40

39

29

20

18

17

16

14

5

2

1

1

1

1
Western Blotting (WB), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

人, 小鼠, 大鼠

预测反应

Dog

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human AQP2.

亚型

IgG
anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 177-271) antibody
AQP2 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 147-271) antibody
AQP2 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 149-271) antibody
AQP2 适用: 人 WB, ELISA, IHC, ICC, FACS, Neut 宿主: 小鼠 Monoclonal 7H8B5 unconjugated

anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 245-260) antibody
AQP2 适用: 人, 大鼠, 小鼠 WB, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Aquaporin 2 (Collecting Duct) (AQP2) (C-Term) antibody
AQP2 适用: 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Aquaporin 2 (Collecting Duct) (AQP2) (pSer261) antibody
AQP2 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Aquaporin 2 (Collecting Duct) (AQP2) (pSer269) antibody
AQP2 适用: 大鼠 WB, ELISA, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 101-200) antibody
AQP2 适用: 人, 大鼠, 小鼠 WB, ELISA, IF (p), IF (cc), IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Aquaporin 2 (Collecting Duct) (AQP2) (C-Term) antibody (HRP)
AQP2 适用: 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal HRP

anti-Aquaporin 2 (Collecting Duct) (AQP2) (C-Term) antibody (FITC)
AQP2 适用: 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal FITC

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

限制

仅限研究用
生效 #029457 (Immunohistochemistry)

by

Immunohistochemistry Core, NYU Langone

No.

#029457

日期

2014.01.16

抗原

Lot Number

999994W

Method validated

Immunohistochemistry

Positive Control

- Human kidney tubules - Human breast ductal epithelium

Negative Control

- Human kidney glomeruli and stromal tissue - Human breast mesenchymal tissue

Notes

Strong signal was observed in positive control tissues and not in negative control tissues.

Validation Images

Figure 1: Human kidney stained with AQP2 (brown).

Figure 2. Human kidney stained with isotype control antibody (brown).

Figure 3: Human kidney stained only with secondary antibody (primary antibody omitted) (brown).

Figure 4: Human breast stained with AQP2 (brown).

Figure 5. Human breast stained with isotype control antibody (brown).

Figure 6: Human breast stained only with secondary antibody (primary antibody omitted) (brown).
Full Methods
Primary Antibody

Antibody: Aquaporin 2
Catalog number: ABIN707576
Batch number: 999994W

Secondary Antibody

Antibody: Biotinylated goat anti-rabbit/anti-mouse (Kit)
Lot number: D05923BA

Full Protocol

Immunohistochemistry was performed on a Ventana NexES automated platform, instrument manufacturer specific reagents are italicized.
1. Slides were preheated in convection oven at 60°C for 30 min
2. Deparaffinization procedure: - 3 changes of Xylene, 5 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of 95% Ethanol, 3 min each - Rinsed in distilled water, 3 changes
3. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 min. - Slides were allowed to cool (in citrate) for 30 min. - Slides were washed x 3 in Distilled water
4. NexES instrument procedure, iVIEW DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
5. Slides rinsed with reaction buffer x3
6. *iVIEW Inhibitor (H2O2)* applied and incubated for 4 min
7. Slides rinsed with *reaction buffer*
8. Antibody Application - Primary antibody diluted 1:250 in PBS (100 µL applied/slide) - Ventana Isotype control applied neat - Slides Incubated overnight at room temperature (~12 hours ~25°C)
9. Slides rinsed with *reaction buffer* x3
10. *iVIEW Biotinylated IgG* applied and incubated for 8 min
11. Slides rinsed with *reaction buffer*
12. *iVIEW Streptavidin-Horseradish Peroxidase* applied and incubated for 8 min
13. Slides rinsed with *reaction buffer*
14. *iVIEW DAB/H2O2* applied and incubated for 8 min
15. Slides rinsed with *reaction buffer*
16. *iVIEW Copper* applied and incubated for 4 min
17. Slides rinsed with *reaction buffer*
18. Slides washed in Dawn Detergent/tap water
19. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 seconds - Slides washed in tap water, 1 min - Decolorized (10% Acetic Acid in 70% ethanol), 1 min - Slides washed in tap water, 1 min - Bluing (Austin Clear Ammonia), 1 min - Slides washed in tap water, 1 min
20. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of Xylene, 5 min each - Mounted with Permount
21. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software

Experimental Notes

Deviations from protocol/procedure supplied by manufacturer.
Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
Step 3.1: Slides heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
Step 3.2: Slides cooled for 30 minutes; manufacturer cools for 20 minutes.
Step 4: Italicized reagents and incubation time are fixed instrument parameters.
Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Lazzeri, Angelotti, Peired, Conte, Marschner, Maggi, Mazzinghi, Lombardi, Melica, Nardi, Ronconi, Sisti, Antonelli, Becherucci, De Chiara, Guevara, Burger, Schaefer, Annunziato, Anders, Lasagni et al.: "Endocycle-related tubular cell hypertrophy and progenitor proliferation recover renal function after acute kidney injury. ..." in: Nature communications, Vol. 9, Issue 1, pp. 1344, (2018) (PubMed).
抗原

AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

别名

AQP2 (AQP2 产品)

别名

AQP-CD antibody, WCH-CD antibody, cph antibody, jpk antibody, AQP-2 antibody, aquaporin-2 antibody, aquaporin 2 antibody, aquaporin 2 (collecting duct) antibody, Aqp2 antibody, AQP2 antibody

背景

Synonyms: AQP-CD, WCH-CD, Aquaporin-2, AQP-2, ADH water channel, Aquaporin-CD, Collecting duct water channel protein, Water channel protein for renal collecting duct, AQP2
Background: Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.

基因ID

359

UniProt

P41181

途径

Response to Water Deprivation