AQP2 抗体 (AA 171-271)
(2 references)
(1 validation)-
- 抗原 See all AQP2 抗体
- AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))
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抗原表位
- AA 171-271
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适用
- 人, 大鼠, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This AQP2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 交叉反应
- 人, 小鼠, 大鼠
- 预测反应
- Dog
- 纯化方法
- Purified by Protein A.
- 免疫原
- KLH conjugated synthetic peptide derived from human AQP2.
- 亚型
- IgG
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anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 177-271) antibody
AQP2 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 147-271) antibodyAQP2 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 149-271) antibodyAQP2 适用: 人 ELISA 宿主: 小鼠 Monoclonal 7H8B5 unconjugated
anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 245-260) antibodyAQP2 适用: 人, 大鼠, 小鼠 WB, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 2 (Collecting Duct) (AQP2) (C-Term) antibodyAQP2 适用: 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 2 (Collecting Duct) (AQP2) (pSer261) antibodyAQP2 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 2 (Collecting Duct) (AQP2) (pSer269) antibodyAQP2 适用: 大鼠 WB, ELISA, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 101-200) antibodyAQP2 适用: 人, 大鼠, 小鼠 WB, ELISA, IF (p), IF (cc), IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 2 (Collecting Duct) (AQP2) (C-Term) antibody (HRP)AQP2 适用: 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal HRP
anti-Aquaporin 2 (Collecting Duct) (AQP2) (C-Term) antibody (FITC)AQP2 适用: 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal FITC
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- 应用备注
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WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500 - 限制
- 仅限研究用
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- by
- Immunohistochemistry Core, NYU Langone
- No.
- #029457
- 日期
- 2014.01.16
- 抗原
- Lot Number
- 999994W
- Method validated
- Immunohistochemistry
- Positive Control
- - Human kidney tubules - Human breast ductal epithelium
- Negative Control
- - Human kidney glomeruli and stromal tissue - Human breast mesenchymal tissue
- Notes
- Strong signal was observed in positive control tissues and not in negative control tissues.
- Primary Antibody
- Antibody: Aquaporin 2
- Catalog number: ABIN707576
- Batch number: 999994W
- Secondary Antibody
- Antibody: Biotinylated goat anti-rabbit/anti-mouse (Kit)
- Lot number: D05923BA
- Full Protocol
- Immunohistochemistry was performed on a Ventana NexES automated platform, instrument manufacturer specific reagents are italicized.
- 1. Slides were preheated in convection oven at 60°C for 30 min
- 2. Deparaffinization procedure: - 3 changes of Xylene, 5 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of 95% Ethanol, 3 min each - Rinsed in distilled water, 3 changes
- 3. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 min. - Slides were allowed to cool (in citrate) for 30 min. - Slides were washed x 3 in Distilled water
- 4. NexES instrument procedure, iVIEW DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
- 5. Slides rinsed with reaction buffer x3
- 6. *iVIEW Inhibitor (H2O2)* applied and incubated for 4 min
- 7. Slides rinsed with *reaction buffer*
- 8. Antibody Application - Primary antibody diluted 1:250 in PBS (100 µL applied/slide) - Ventana Isotype control applied neat - Slides Incubated overnight at room temperature (~12 hours ~25°C)
- 9. Slides rinsed with *reaction buffer* x3
- 10. *iVIEW Biotinylated IgG* applied and incubated for 8 min
- 11. Slides rinsed with *reaction buffer*
- 12. *iVIEW Streptavidin-Horseradish Peroxidase* applied and incubated for 8 min
- 13. Slides rinsed with *reaction buffer*
- 14. *iVIEW DAB/H2O2* applied and incubated for 8 min
- 15. Slides rinsed with *reaction buffer*
- 16. *iVIEW Copper* applied and incubated for 4 min
- 17. Slides rinsed with *reaction buffer*
- 18. Slides washed in Dawn Detergent/tap water
- 19. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 seconds - Slides washed in tap water, 1 min - Decolorized (10% Acetic Acid in 70% ethanol), 1 min - Slides washed in tap water, 1 min - Bluing (Austin Clear Ammonia), 1 min - Slides washed in tap water, 1 min
- 20. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of Xylene, 5 min each - Mounted with Permount
- 21. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software
- Experimental Notes
- Deviations from protocol/procedure supplied by manufacturer.
- Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
- Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
- Step 3.1: Slides heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
- Step 3.2: Slides cooled for 30 minutes; manufacturer cools for 20 minutes.
- Step 4: Italicized reagents and incubation time are fixed instrument parameters.
- Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
生效 #029457 (Immunohistochemistry)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Figure 1: Human kidney stained with AQP2 (brown).]()
Figure 1: Human kidney stained with AQP2 (brown).
![Figure 2. Human kidney stained with isotype control antibody (brown).]()
Figure 2. Human kidney stained with isotype control antibody (brown).
![Figure 3: Human kidney stained only with secondary antibody (primary antibody omitted) (brown).]()
Figure 3: Human kidney stained only with secondary antibody (primary antibody omitted) (brown).
![Figure 4: Human breast stained with AQP2 (brown).]()
Figure 4: Human breast stained with AQP2 (brown).
![Figure 5. Human breast stained with isotype control antibody (brown).]()
Figure 5. Human breast stained with isotype control antibody (brown).
![Figure 6: Human breast stained only with secondary antibody (primary antibody omitted) (brown).]()
Figure 6: Human breast stained only with secondary antibody (primary antibody omitted) (brown).
Full Methods -
- 状态
- Liquid
- 浓度
- 1 μg/μL
- 缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- 有效期
- 12 months
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: "Endocycle-related tubular cell hypertrophy and progenitor proliferation recover renal function after acute kidney injury. ..." in: Nature communications, Vol. 9, Issue 1, pp. 1344, (2018) (PubMed).
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: "Endocycle-related tubular cell hypertrophy and progenitor proliferation recover renal function after acute kidney injury. ..." in: Nature communications, Vol. 9, Issue 1, pp. 1344, (2018) (PubMed).
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- 抗原
- AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))
- 别名
- AQP2 (AQP2 产品)
- 别名
- AQP-CD antibody, WCH-CD antibody, cph antibody, jpk antibody, AQP-2 antibody, aquaporin-2 antibody, aquaporin 2 antibody, aquaporin 2 (collecting duct) antibody, Aqp2 antibody, AQP2 antibody
- 背景
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Synonyms: AQP-CD, WCH-CD, Aquaporin-2, AQP-2, ADH water channel, Aquaporin-CD, Collecting duct water channel protein, Water channel protein for renal collecting duct, AQP2
Background: Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
- 基因ID
- 359
- UniProt
- P41181
- 途径
- Response to Water Deprivation
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