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AQP2 抗体 (AA 171-271)

AQP2 适用: 人, 大鼠, 小鼠 WB, ELISA, IF (p), IF (cc), IHC (p), ICC, FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN707576
发货至: 中国
  • 抗原 See all AQP2 抗体
    AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))
    抗原表位
    • 21
    • 20
    • 17
    • 16
    • 15
    • 15
    • 7
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 171-271
    适用
    • 108
    • 95
    • 79
    • 15
    • 15
    • 10
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    人, 大鼠, 小鼠
    宿主
    • 130
    • 2
    克隆类型
    • 131
    • 2
    多克隆
    标记
    • 46
    • 10
    • 9
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This AQP2 antibody is un-conjugated
    应用范围
    • 115
    • 40
    • 40
    • 39
    • 29
    • 20
    • 18
    • 17
    • 16
    • 14
    • 5
    • 2
    • 1
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    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
    交叉反应
    人, 小鼠, 大鼠
    预测反应
    Dog
    纯化方法
    Purified by Protein A.
    免疫原
    KLH conjugated synthetic peptide derived from human AQP2.
    亚型
    IgG
  • 应用备注
    WB 1:300-5000
    ELISA 1:500-1000
    FCM 1:20-100
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    ICC 1:100-500
    限制
    仅限研究用
  • 生效 #029457 (Immunohistochemistry)
    '独立验证'标志
    by
    Immunohistochemistry Core, NYU Langone
    No.
    #029457
    日期
    2014.01.16
    抗原
    Lot Number
    999994W
    Method validated
    Immunohistochemistry
    Positive Control
    - Human kidney tubules - Human breast ductal epithelium
    Negative Control
    - Human kidney glomeruli and stromal tissue - Human breast mesenchymal tissue
    Notes
    Strong signal was observed in positive control tissues and not in negative control tissues.
    '独立验证'标志
    Validation Images
    Full Methods
    Primary Antibody
    • Antibody: Aquaporin 2
    • Catalog number: ABIN707576
    • Batch number: 999994W
    Secondary Antibody
    • Antibody: Biotinylated goat anti-rabbit/anti-mouse (Kit)
    • Lot number: D05923BA
    Full Protocol
    • Immunohistochemistry was performed on a Ventana NexES automated platform, instrument manufacturer specific reagents are italicized.
    • 1. Slides were preheated in convection oven at 60°C for 30 min
    • 2. Deparaffinization procedure: - 3 changes of Xylene, 5 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of 95% Ethanol, 3 min each - Rinsed in distilled water, 3 changes
    • 3. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 min. - Slides were allowed to cool (in citrate) for 30 min. - Slides were washed x 3 in Distilled water
    • 4. NexES instrument procedure, iVIEW DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
    • 5. Slides rinsed with reaction buffer x3
    • 6. *iVIEW Inhibitor (H2O2)* applied and incubated for 4 min
    • 7. Slides rinsed with *reaction buffer*
    • 8. Antibody Application - Primary antibody diluted 1:250 in PBS (100 µL applied/slide) - Ventana Isotype control applied neat - Slides Incubated overnight at room temperature (~12 hours ~25°C)
    • 9. Slides rinsed with *reaction buffer* x3
    • 10. *iVIEW Biotinylated IgG* applied and incubated for 8 min
    • 11. Slides rinsed with *reaction buffer*
    • 12. *iVIEW Streptavidin-Horseradish Peroxidase* applied and incubated for 8 min
    • 13. Slides rinsed with *reaction buffer*
    • 14. *iVIEW DAB/H2O2* applied and incubated for 8 min
    • 15. Slides rinsed with *reaction buffer*
    • 16. *iVIEW Copper* applied and incubated for 4 min
    • 17. Slides rinsed with *reaction buffer*
    • 18. Slides washed in Dawn Detergent/tap water
    • 19. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 seconds - Slides washed in tap water, 1 min - Decolorized (10% Acetic Acid in 70% ethanol), 1 min - Slides washed in tap water, 1 min - Bluing (Austin Clear Ammonia), 1 min - Slides washed in tap water, 1 min
    • 20. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of Xylene, 5 min each - Mounted with Permount
    • 21. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software
    Experimental Notes
    • Deviations from protocol/procedure supplied by manufacturer.
    • Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
    • Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
    • Step 3.1: Slides heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
    • Step 3.2: Slides cooled for 30 minutes; manufacturer cools for 20 minutes.
    • Step 4: Italicized reagents and incubation time are fixed instrument parameters.
    • Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
  • 状态
    Liquid
    浓度
    1 μg/μL
    缓冲液
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    储存液
    ProClin
    注意事项
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    有效期
    12 months
  • Lazzeri, Angelotti, Peired, Conte, Marschner, Maggi, Mazzinghi, Lombardi, Melica, Nardi, Ronconi, Sisti, Antonelli, Becherucci, De Chiara, Guevara, Burger, Schaefer, Annunziato, Anders, Lasagni et al.: "Endocycle-related tubular cell hypertrophy and progenitor proliferation recover renal function after acute kidney injury. ..." in: Nature communications, Vol. 9, Issue 1, pp. 1344, (2018) (PubMed).

  • 抗原
    AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))
    别名
    AQP2 (AQP2 产品)
    别名
    AQP-CD antibody, WCH-CD antibody, cph antibody, jpk antibody, AQP-2 antibody, aquaporin-2 antibody, aquaporin 2 antibody, aquaporin 2 (collecting duct) antibody, Aqp2 antibody, AQP2 antibody
    背景

    Synonyms: AQP-CD, WCH-CD, Aquaporin-2, AQP-2, ADH water channel, Aquaporin-CD, Collecting duct water channel protein, Water channel protein for renal collecting duct, AQP2

    Background: Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.

    基因ID
    359
    UniProt
    P41181
    途径
    Response to Water Deprivation
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