Recombinant CCRL2 抗体 (AA 1-24)
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- 抗原 See all CCRL2 抗体
- CCRL2 (Chemokine (C-C Motif) Receptor-Like 2 (CCRL2))
- 抗体类型
- Recombinant Antibody
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抗原表位
- AA 1-24
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- Chimeric
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标记
- This CCRL2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Functional Studies (Func), Immunoprecipitation (IP)
- 原理
- Anti-CCRL2 [BZ5B8], Rabbit IgG, kappa
- 特异性
- This antibody is specific for murine CCRL2 (formerly known as L-CCR), which is expressed on mast cells, activated macrophages, and endothelial cells. This receptor binds the leukocyte chemoattractant chemerin without triggering classical GPCR signaling, providing a specific mechanism for local enrichment of chemerin at inflammatory sites. CCRL2 presents active chemerin to leukocytes expressing the chemoattractant receptor CMKLR1/Chemr23. Chemerin can block anti-CCRL2 antibody binding.
- 产品特性
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Original Species of Ab: Rat
Original Format of Ab: IgG2a
- 纯化方法
- Protein A affinity purified
- 免疫原
- This antibody was raised by immunising Wistar Furth rats with the mCCRL2 peptide/KLH conjugate. The immunizing amino-terminal mCCRL2 peptide with the sequence NH2-MDNYTVAPDDEYDVLILDDYLDNSC-COOH, corresponding to residues 1-24 of mCCRL2, with a nonnative carboxyl-terminal cysteine to facilitate conjugation to keyhole limpet hemocyanin (KLH).
- 克隆位点
- BZ5B8
- 亚型
- IgG kappa
- Top Product
- Discover our top product CCRL2 Primary Antibody
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anti-Chemokine (C-C Motif) Receptor-Like 2 (CCRL2) antibody
CCRL2 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Receptor-Like 2 (CCRL2) (AA 1-43) antibodyCCRL2 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Receptor-Like 2 (CCRL2) (Cytoplasmic Domain) antibodyCCRL2 适用: 人 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Receptor-Like 2 (CCRL2) (AA 1-344) antibodyCCRL2 适用: 人 WB, FACS 宿主: 小鼠 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Receptor-Like 2 (CCRL2) (Internal Region) antibodyCCRL2 适用: 人, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Receptor-Like 2 (CCRL2) (Internal Region) antibodyCCRL2 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Receptor-Like 2 (CCRL2) (AA 1-344) antibodyCCRL2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Receptor-Like 2 (CCRL2) (AA 36-85) antibodyCCRL2 适用: 人, Cow, 豚鼠, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Receptor-Like 2 (CCRL2) (AA 141-190) antibodyCCRL2 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Receptor-Like 2 (CCRL2) (Internal Region) antibodyCCRL2 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- This antibody has been used in various FACS analyses, for instance, to confirm that it is only specific to mCCRL2-hemagglutinin (HA)/L1.2 transfectants and that peritoneal macrophages treated with LPS, TNFα, IFN-γ, or poly:IC up-regulate mCCRL2 protein expression (Zabel et al., 2008). Of note, this antibody binding to mouse peritoneal mast cells could be inhibited by chemerin, a protein ligand for signaling receptor CMKLR1 (Zabel et al., 2008). This anti-CCRL2 antibody can be used in wide variety of assays to study leukocytes and immune response. For example, this antibody has been used to examine the expression, regulation, and function of atypical chemerin receptor CCRL2 on endothelial cells (Monnier et al., 2012) and to delineate how the chemoattractant chemerin suppresses melanoma by recruiting natural killer cell antitumor defenses (Pachynski et al., 2012).
- 说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
- 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- PBS with 0.02 % Proclin 300.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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