Recombinant HAVCR1 抗体
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- 抗原 See all HAVCR1 抗体
- HAVCR1 (Hepatitis A Virus Cellular Receptor 1 (HAVCR1))
- 抗体类型
- Recombinant Antibody
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- Chimeric
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标记
- This HAVCR1 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Agonist (Agon)
- 原理
- Anti-Tim-1 [3B3], Rabbit IgG, kappa
- 特异性
- This antibody is specific for murine TIM-1, a member of the T cell immunoglobulin domain, mucin-like domain (TIM) gene family. TIM-1 has been found on activated CD4+ T cells, mast cells, and a subset of B cells. TIM-1 is involved in renal injury, tissue homeostasis, and T cell costimulation, and promotes the differentiation of Th2 cells and the production of IL-4. TIM-1 can bind several ligands, including Hepatitis A virus, phosphatidylserine, TIM4, IgA, as well as itself.
- 产品特性
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Original Species of Ab: Rat
Original Format of Ab: IgG2a
- 纯化方法
- Protein A affinity purified
- 免疫原
- This antibody was raised by immunising female Lewis strain rats (Harlan Sprague-Dawley) subcutaneously with mouse TIM-1-Ig in complete Freund's adjuvant (CFA), followed by multiple 'boosting' with mouse TIM-1-Ig in PBS.
- 克隆位点
- 3B3
- 亚型
- IgG kappa
- Top Product
- Discover our top product HAVCR1 Primary Antibody
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anti-Hepatitis A Virus Cellular Receptor 1 (HAVCR1) (AA 332-348), (C-Term) antibody
HAVCR1 适用: 人 WB, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Hepatitis A Virus Cellular Receptor 1 (HAVCR1) (AA 21-240) antibodyHAVCR1 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C2 unconjugated
anti-Hepatitis A Virus Cellular Receptor 1 (HAVCR1) (AA 22-235) antibodyHAVCR1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Hepatitis A Virus Cellular Receptor 1 (HAVCR1) (AA 1-364) antibodyHAVCR1 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 2G11 unconjugated
anti-Hepatitis A Virus Cellular Receptor 1 (HAVCR1) (AA 70-290) antibodyHAVCR1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3D9F5 unconjugated
anti-Hepatitis A Virus Cellular Receptor 1 (HAVCR1) (AA 70-290) antibodyHAVCR1 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 3A12E10 unconjugated
anti-Hepatitis A Virus Cellular Receptor 1 (HAVCR1) (AA 21-240) antibodyHAVCR1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Hepatitis A Virus Cellular Receptor 1 (HAVCR1) (AA 21-293) antibodyHAVCR1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Hepatitis A Virus Cellular Receptor 1 (HAVCR1) (AA 22-235) antibodyHAVCR1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Hepatitis A Virus Cellular Receptor 1 (HAVCR1) (AA 21-290) antibodyHAVCR1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- This antibody has been used in multiple FACS analyses, such as to determine if specific B cell subsets or if B cell-derived interleukin-10 contributes to tolerance (Lai et al, 2015), to test if blocking several checkpoint receptors boosts anti-tumor immunity in a low-dose, lympho-depleting whole body irradiation model (Jing et al, 2015), and to study the functions of kidney pericytes in vascular stability (Schrimpf et al, 2012). This antibody has also been used in immunohistochemistry to investigate the distinct role of matrix metalloproteinase-3 in TIM-1 shedding by kidney proximal tubular epithelial cells (Lim et al. 2012), and to demonstrate how Bβ(15-42) attenuates the effect of ischemia-reperfusion injury in renal transplantation (Sörensen et al, 2011). In addition, in vivo applications of this agonistic anti-TIM-1 antibody (clone 3B3) has been shown, for instance, to heighten T cell activation and prevent the development of respiratory tract tolerance in a Th2-driven model of asthma (Umetsu et al, 2005), to increase the frequency of antigen-specific T cells, the production of the proinflammatory cytokines IFN-γ and IL-17, and thus the severity of experimental autoimmune encephalomyelitis (Sheng et al, 2007), as well as to deprogram Tregs and prevent transplant tolerance in mice (Degauque et al, 2008). The agonistic effect of 3B3 is exerted by cross-linking of TIM-1 Molecules, as Fab fragments of 3B3 do not show this activity (Umetsu, 2005). As this cross-linking was observed in the presence of dendritic cells, using a rat IgG2a version of theantibody, the cross-linking is likely Fc-dependet.
- 说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
- 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- PBS with 0.02 % Proclin 300.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- 抗原
- HAVCR1 (Hepatitis A Virus Cellular Receptor 1 (HAVCR1))
- 别名
- Tim-1 (HAVCR1 产品)
- 物质类
- Virus
- 背景
- CD365, HAVcr-1, HAVCR1, Hepatitis A virus cellular receptor 1 homolog, Kidney injury molecule 1, KIM-1, T cell immunoglobulin and mucin domain-containing protein 1, T cell membrane protein 1, T-cell immunoglobulin and mucin domain containing 1, T-cell immunoglobulin mucin receptor 1, TIM-1, TIM1, TIMD-1
- UniProt
- Q5QNS5
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