Recombinant MHC, Class I 抗体
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- 抗原 See all MHC, Class I 抗体
- MHC, Class I
- 抗体类型
- Recombinant Antibody
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- Chimeric
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标记
- This MHC, Class I antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunoassay (IA)
- 原理
- Anti-MHC I [R1-21.2], Rabbit IgG, kappa
- 特异性
- This antibody is specific for murine MHC class I, and binds to polymorphic H-2 determinants expressed on most haplotypes. This antibody is, therefore, able to bind all H-2 class I molecules, independent of the allele. This antibody inhibits the binding of most mouse anti-H-2 antibodies to H-2k cells.
- 产品特性
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Original Species of Ab: Rat
Original Format of Ab: IgG2b
- 纯化方法
- Protein A affinity purified
- 免疫原
- This antibody was raised by immunising Lewis 1F rats with mouse CH-1 lymphoma cells. Spleen cells from immunised rats were fused with X-63Ag8-653 myeloma cells to generate hybridomas.
- 克隆位点
- R1-21-2
- 亚型
- IgG kappa
- Top Product
- Discover our top product MHC, Class I Primary Antibody
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anti-MHC, Class I antibody
适用: 人, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-MHC, Class I antibody适用: 绵羊, Cat FACS, WB, IP, IHC (fro) 宿主: 小鼠 Monoclonal VPM19 unconjugated
anti-MHC, Class I (AA 81-180) antibody适用: 人 WB, ELISA, IHC (fro), IF (cc), IF (p), IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-MHC, Class I antibody适用: 小鼠 IP, IF, IA 宿主: 兔 Chimeric R1-9-6 unconjugated Recombinant Antibody
anti-MHC, Class I antibody (PE)适用: 小鸡 FACS, IHC 宿主: 小鼠 Monoclonal F21-2 PE
anti-MHC, Class I antibody适用: 小鸡 FACS, WB, IHC, IP 宿主: 小鼠 Monoclonal F21-2 unconjugated
anti-MHC, Class I antibody (Biotin)适用: 小鸡 FACS, WB, IHC, IP 宿主: 小鼠 Monoclonal F21-2 Biotin
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- 应用备注
- This antibody binds murine H-2 MHC class I molecules, and has been used in both structural and biochemical studies. It has been employed to assess MHC class I expression in frozen, acetone-fixed mouse brain sections by immunohistochemistry (Miralvès et al, 2007), and to stain paraffin-embedded sections of mouse liver for H-2Kb expression (Limmer et al, 2005). It has also been used to precipitate mouse H-2Kk and H-2Dk class I molecules from detergent lysates of Ltk3.3/1 cells (Rada et al, 1990), and to stain H-2Kb and H-2Kk-expressing cells in FACS analysis (Bendelac, 1995). Cellular radioimmune assays have been used to confirm the binding of this antibody to spleen cells, and assess its strain specificity (Koch et al, 1983).
- 说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original format, for improved compatibility with existing reagents, assays and techniques.
- 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- PBS with 0.02 % Proclin 300.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- 抗原
- MHC, Class I
- 别名
- MHC I (MHC, Class I 产品)
- 背景
- R1.21.2, H-2, MHC-I, MHC Class I
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