Recombinant Desmosome/hemidesmosome 抗体
适用: 人
IEM, IF
宿主: 兔
Chimeric
F12
unconjugated
Recombinant Antibody
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- 抗原
- Desmosome/hemidesmosome
- 抗体类型
- Recombinant Antibody
- 适用
- 人
-
宿主
- 兔
-
克隆类型
- Chimeric
-
标记
- 非结合性
- 应用范围
- Immunoelectron Microscopy (IEM), Immunofluorescence (IF)
- 原理
- Anti-desmosome/hemidesmosome [F12], Rabbit IgG, kappa
- 特异性
- F12 recognizes an unknown polypeptide of desmosomal and hemidesmosomal plaques with a MW of 185 kDa (determined from immunoblotting using bovine tongue epithelium extract as the substrate), and by IEM (immunoelectron microscopy) it was determined that it is the intracellular part of the desmosome that is bound (Gilbert, 1997). F12 shares some immunochemical properties with autoantibodies present in paraneoplastic pemphigus sera. Pemphigus diseases are those resulting in blistering of the skin due to the production of autoantibodies against keratinocyte surface proteins - autoantibodies of patients with pemphius vulgaris recognize desmoglein 1 (Dsg1), a major glycoprotein of the desmosome complex.
- 产品特性
-
Original Species of Ab: Human
Original Format of Ab: IgM
- 纯化方法
- Protein A affinity purified
- 免疫原
- F12 was established by fusing peripheral blood lymphocytes derived from a human vulgaris patient with a hetermyeloma cell line.
- 克隆位点
- F12
- 亚型
- IgG kappa
-
-
- 应用备注
- F12 labels the keratinocyte membrane and the basement membrane zone of rat tongue sections by indirect immunofluorescence (IIF). The antibody also stains both the cell membrane and the basement membrane zone of stratified squamous epithelia, and other epithelial tissues such as urinary bladder, small bowel, thymus, and liver. Non-epithelial tissues, such as myocardium are additionally stained. Sera from patients with pemphigus vulgaris can be incubated with human skin sections to determine their capacity to block the binding of F12 in an IIF blocking assay. IEM (immunoelectron microscopy) can be used to determine the ultralocalization of the antibody.
- 说明
-
This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgM format, for improved compatibility with existing reagents, assays and techniques.
- 限制
- 仅限研究用
-
- 浓度
- 1 mg/mL
- 缓冲液
- PBS with 0.02 % Proclin 300.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
-
- 抗原
- Desmosome/hemidesmosome
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