Recombinant EDAR 抗体
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- 抗原 See all EDAR 抗体
- EDAR (Ectodysplasin A Receptor (EDAR))
- 抗体类型
- Recombinant Antibody
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适用
- 人, 小鼠, 大鼠, 犬, 小鸡
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宿主
- 兔
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克隆类型
- Chimeric
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标记
- This EDAR antibody is un-conjugated
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应用范围
- ELISA, Western Blotting (WB), Surface Plasmon Resonance (SPR), Agonist (Agon)
- 原理
- Anti-EDAR [EDAR12], Rabbit IgG, kappa
- 特异性
- EDAR12 recognises and binds to CRD1 of the extracellular domain of mouse EDAR. EDAR3 cross-reacts with EDAR derived from human, dog, rat and chicken when EDAR is fused to the glycosylphosphatidylinositol anchor of TRAILR3. EDAR is the receptor for the TNF family ligand EDA1, which is a type II transmembrane protein possessing a collagen-like domain and a C-terminal TNF-homology domain. EDAR is important for the correct development of skin appendages including hair, teeth and eccrine sweat glands. LoF mutations in the Eda gene is known to cause XLHED (X-linked hypohidrotic ectodermal dysplasia), and results in abnormal development.
- 交叉反应
- 小鸡, 犬, 小鼠, 大鼠
- 产品特性
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Original Species of Ab: Mouse
Original Format of Ab: IgG1
- 纯化方法
- Protein A affinity purified
- 免疫原
- EDAR12 was prepared by immunixzing female OVE1B mice (with the Edar gene deleted) subcutaneously with mouse EDAR-Fc and positive hybridoma clones were screened for binding mEDAR by ELISA.
- 克隆位点
- EDAR12
- 亚型
- IgG kappa
- Top Product
- Discover our top product EDAR Primary Antibody
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anti-Ectodysplasin A Receptor (EDAR) (AA 27-170) antibody
EDAR 适用: 人 ELISA, WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ectodysplasin A Receptor (EDAR) (AA 64-178) antibodyEDAR 适用: 人 ELISA, WB 宿主: 小鼠 Polyclonal unconjugated
anti-Ectodysplasin A Receptor (EDAR) (Middle Region) antibodyEDAR 适用: 人, 小鼠, 大鼠, 犬, Cow, 马, 豚鼠, 兔 WB 宿主: 兔 Polyclonal unconjugated
anti-Ectodysplasin A Receptor (EDAR) antibodyEDAR 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ectodysplasin A Receptor (EDAR) antibodyEDAR 适用: 人, 小鼠, 大鼠, 犬, 小鸡 ELISA, WB, SPR, Agon 宿主: 小鼠 Monoclonal EDAR12 unconjugated Recombinant Antibody
anti-Ectodysplasin A Receptor (EDAR) (AA 64-178) antibodyEDAR 适用: 人 ELISA, WB 宿主: 小鼠 Monoclonal 3A7 unconjugated
anti-Ectodysplasin A Receptor (EDAR) (AA 290-339) antibodyEDAR 适用: 人, 小鼠, 大鼠, 犬, Cow, 马, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Ectodysplasin A Receptor (EDAR) (AA 27-187) antibodyEDAR 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ectodysplasin A Receptor (EDAR) (Middle Region) antibodyEDAR 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- EDAR12 can be used in surrogate reporter assay in which Fas-sensitive cells were transfected with a construct formed by fusing the ectodomain of mouse or human EDAR to the intracellular domain of Fas. Binding of EDAR12 resulted in the induction of apoptosis in these cells, confirming the agonisitc activity of the antibody. (EC50 (dose required to kill half of EDAR:Fas-expressing Jurkat cells) hEDAR:Fas ~ 5 ng/mL, EC50 mEDAR:Fas ~ 10 ng/mL - both were less active than DEA1-Fc). Tail-hair formation was also rescued after administration of EDAR3 to newborn EDA-deficient Tabby mice, with 0.35 mg/kg required for half-maximal tail-hair reversion. EDAR123 can also be used in ELISA and as a Fab fragment (generated by ficin digestion) in SPR experiments. EDAR can also be recognized by EDAR12 under both reducing (+DTT) and non-reducing conditions.
- 说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
- 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- PBS with 0.02 % Proclin 300.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- 抗原
- EDAR (Ectodysplasin A Receptor (EDAR))
- 别名
- EDAR (EDAR 产品)
- 背景
- Ectodysplasin A receptor, Tumor necrosis factor receptor superfamily member EDAR, Anhidrotic ectodysplasin receptor 1, Downless, Ectodermal dysplasia receptor, Ectodysplasin-A receptor
- UniProt
- Q9R187
- 途径
- Tube Formation, Ubiquitin Proteasome Pathway
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