The mouse monoclonal antibody CLB-GB11 recognizes granzyme B, a 31 kDa serine protease expressed intracellularly in activated Tc cells and NK cells.
纯化方法
Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular staining.
Granzyme B,Granzyme B is a serine protease that is expressed in cytoplasmic granules of cytotoxic T lymphocytes and NK cells. Vectorial secretion of perforin and granzymes is responsible for their granule-mediated cytotoxicity. Granzyme B plays a pivotal role in the induction of apoptosis in the target cells by activation of caspases. Moreover, granzyme B was reported to cleave directly alpha-tubulin, leading to perturbation of microtubule networks during the induced cell death.,GZMB, HLP, CTLA1, SECT