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DDX4 抗体 (AA 601-700)

Name: DDX4 抗体 (AA 601-700)
SKU: ABIN701365
Availability: OutOfStock

DDX4 适用: 小鸡 WB, FACS, IHC (p), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Formalin-fixed and paraffin embedded rat ovarian tissue labeled with Anti-DDX4/MVH Polyclonal Antibody, Unconjugated (ABIN701365) at 1:300 followed by conjugation to the secondary antibody and DAB staining (DDX4 抗体 (AA 601-700))

IHC

Flow cytometry analysis of mesenchymal stem cells derived from bone marrow (upper) and adipose tissue-derived mesenchymal stem cells (lower) after culturing in the medium contains bone morphogenic protein-4 for 4 days. (DDX4 抗体 (AA 601-700))

FACS

Paraformaldehyde-fixed, paraffin embedded mouse testis, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with DDX4 Polyclonal Antibody (bs-3597R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining. (DDX4 抗体 (AA 601-700))

IHC (p)

3 images

(2 references)

产品编号 ABIN701365

发货至: 中国

Datasheet as PDF

抗原 See all DDX4 抗体

DDX4 (DEAD Box Protein 4 (DDX4))
抗原表位
15

7

7

6

5

5

3

2

2

2

2

1

1

1

1

1

1
AA 601-700
适用
53

24

15

11

2

2

1

1

1

1

1

1

1

1
小鸡
宿主
65

8
兔
Compatible Secondaries
克隆类型
67

6
多克隆
标记
29

6

5

5

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
This DDX4 antibody is un-conjugated
应用范围
51

23

15

12

11

10

5

4

4

2

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

小鸡

预测反应

Human,Mouse,Rat,Dog,Cow,Pig,Rabbit

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human DDX4

亚型

IgG

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anti-DEAD Box Protein 4 (DDX4) antibody
DDX4 适用: 人 ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 2F9H5 unconjugated

anti-DEAD Box Protein 4 (DDX4) (AA 253-272), (Middle Region) antibody
DDX4 适用: 人, 小鼠, 大鼠 WB, IHC (p), IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated

anti-DEAD Box Protein 4 (DDX4) (AA 1-300) antibody
DDX4 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-DEAD Box Protein 4 (DDX4) (AA 1-223) antibody
DDX4 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-DEAD Box Protein 4 (DDX4) (AA 625-724) antibody
DDX4 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3D5 unconjugated

anti-DEAD Box Protein 4 (DDX4) antibody
DDX4 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-DEAD Box Protein 4 (DDX4) (AA 601-700) antibody (FITC)
DDX4 适用: 小鸡 WB, FACS, IF (p) 宿主: 兔 Polyclonal FITC

anti-DEAD Box Protein 4 (DDX4) (AA 1-690) antibody
DDX4 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated

anti-DEAD Box Protein 4 (DDX4) (AA 700-724) antibody
DDX4 适用: 人, 小鼠, 马, 猴, 犬 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-DEAD Box Protein 4 (DDX4) (AA 554-583), (C-Term) antibody
DDX4 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB13974 unconjugated

应用备注

WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Shirzeyli, Tayyebiazar, Aliakbari, Ghasemi, Eini, Shirzeyli, Vanaki, Sobhani: "Comparison of the efficacy of bone morphogenetic protein-4 on in vitro differentiation of murine adipose and bone marrow mesenchymal stem cells into primordial germ cells." in: , Vol. 17, Issue 2, pp. 123-133, (2022) (PubMed).

Imus, Roe, Charter, Durrant, Jensen: "Transfer and detection of freshly isolated or cultured chicken (Gallus gallus) and exotic species' embryonic gonadal germ stem cells in host embryos." in: Zoological science, Vol. 31, Issue 6, pp. 360-8, (2014) (PubMed).
抗原

DDX4 (DEAD Box Protein 4 (DDX4))

别名

DDX4 (DDX4 产品)

别名

VASA antibody, AV206478 antibody, Mvh antibody, RVLG antibody, vasa antibody, vlg1 antibody, DEAD-box helicase 4 antibody, DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 antibody, DEAD-box helicase 4 L homeolog antibody, DDX4 antibody, Ddx4 antibody, ddx4.L antibody

背景

Synonyms: VASA, Probable ATP-dependent RNA helicase DDX4, DEAD box protein 4, Vasa homolog, DDX4
Background: Summary: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

基因ID

54514

UniProt

Q9NQI0