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MBP Tag 抗体

WB 宿主: 小鼠 Monoclonal 8K2 unconjugated
产品编号 ABIN7250836
发货至: 中国
  • 抗原
    MBP Tag
    宿主
    • 12
    • 5
    小鼠
    克隆类型
    • 11
    • 5
    单克隆
    标记
    • 15
    • 1
    • 1
    非结合性
    应用范围
    • 15
    • 8
    • 7
    • 5
    • 3
    • 1
    • 1
    Western Blotting (WB)
    产品特性
    Tag Antibody
    纯化方法
    Protein A purification
    免疫原
    Recombinant Protein
    克隆位点
    8K2
    亚型
    IgG
  • 应用备注
    WB 1:5000-1:10000
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    PBS with 0.02 % sodium azide and 50 % glycerol pH 7.4.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    Store at -20°C. Avoid freeze / thaw cycles.
  • 抗原
    MBP Tag
    别名
    MBP-Tag
    物质类
    Tag
    背景
    Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site.
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